Regulation of LANCEOLATE by miR319 is required for compound-leaf development in tomato

Naomi Ori; Aya Refael Cohen; Adi Etzioni; Arnon Brand; Osnat Yanai; Sharona Shleizer; Naama Menda; Ziva Amsellem; Idan Efroni; Irena Pekker; John Paul Alvarez; Eyal Blum; Dani Zamir; Yuval Eshed

doi:10.1038/ng2036

Regulation of LANCEOLATE by miR319 is required for compound-leaf development in tomato

Naomi Ori^*, Aya Refael Cohen, Adi Etzioni, Arnon Brand, Osnat Yanai, Sharona Shleizer, Naama Menda, Ziva Amsellem, Idan Efroni, Irena Pekker, John Paul Alvarez, Eyal Blum, Dani Zamir, Yuval Eshed

^*Corresponding author for this work

Institute of Plant Sciences and Genetics in Agriculture

Research output: Contribution to journal › Article › peer-review

449 Scopus citations

Abstract

Plant leaves show pronounced plasticity of size and form. In the classical, partially dominant mutation Lanceolate (La), the large compound leaves of tomato (Solanum lycopersicum) are converted into small simple ones. We show that LA encodes a transcription factor from the TCP family containing an miR319-binding site. Five independent La isolates are gain-of-function alleles that result from point mutations within the miR319-binding site and confer partial resistance of the La transcripts to microRNA (miRNA)-directed inhibition. The reduced sensitivity to miRNA regulation leads to elevated LA expression in very young La leaf primordia and to precocious differentiation of leaf margins. In contrast, downregulation of several LA-like genes using ectopic expression of miR319 resulted in larger leaflets and continuous growth of leaf margins. Our results imply that regulation of LA by miR319 defines a flexible window of morphogenetic competence along the developing leaf margin that is required for leaf elaboration.

Original language	English
Pages (from-to)	787-791
Number of pages	5
Journal	Nature Genetics
Volume	39
Issue number	6
DOIs	https://doi.org/10.1038/ng2036
State	Published - Jun 2007

Bibliographical note

Funding Information:
We wish to thank E. Lifschitz5 (Technion), the C.M. Rick Tomato Genetics Resource Center and Clemson University Genomics Institute for materials, Navot Ori for unearthing la-6, and R. Fluhr, E. Hornstein, Z. Lippman, D. Weigel, S. Hake, J. Bowman and members of our laboratories for discussions and criticism. The work was supported by grants from the US-Israel Binational Agricultural Research and Development Fund, the European Union (MechPlant project) and the Israel Science Foundation (ISF) to N.O.; from ISF, MINERVA, The US-Israel Binational Science Foundation and Lubin Center for Plant Biotechnology to Y.E.; from the German-Israeli Foundation for Scentific Research and Development to Y.E. and N.O.; and from the German-Israeli Project Cooperation to Y.E. and D.Z.

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title = "Regulation of LANCEOLATE by miR319 is required for compound-leaf development in tomato",

abstract = "Plant leaves show pronounced plasticity of size and form. In the classical, partially dominant mutation Lanceolate (La), the large compound leaves of tomato (Solanum lycopersicum) are converted into small simple ones. We show that LA encodes a transcription factor from the TCP family containing an miR319-binding site. Five independent La isolates are gain-of-function alleles that result from point mutations within the miR319-binding site and confer partial resistance of the La transcripts to microRNA (miRNA)-directed inhibition. The reduced sensitivity to miRNA regulation leads to elevated LA expression in very young La leaf primordia and to precocious differentiation of leaf margins. In contrast, downregulation of several LA-like genes using ectopic expression of miR319 resulted in larger leaflets and continuous growth of leaf margins. Our results imply that regulation of LA by miR319 defines a flexible window of morphogenetic competence along the developing leaf margin that is required for leaf elaboration.",

author = "Naomi Ori and Cohen, {Aya Refael} and Adi Etzioni and Arnon Brand and Osnat Yanai and Sharona Shleizer and Naama Menda and Ziva Amsellem and Idan Efroni and Irena Pekker and Alvarez, {John Paul} and Eyal Blum and Dani Zamir and Yuval Eshed",

note = "Funding Information: We wish to thank E. Lifschitz5 (Technion), the C.M. Rick Tomato Genetics Resource Center and Clemson University Genomics Institute for materials, Navot Ori for unearthing la-6, and R. Fluhr, E. Hornstein, Z. Lippman, D. Weigel, S. Hake, J. Bowman and members of our laboratories for discussions and criticism. The work was supported by grants from the US-Israel Binational Agricultural Research and Development Fund, the European Union (MechPlant project) and the Israel Science Foundation (ISF) to N.O.; from ISF, MINERVA, The US-Israel Binational Science Foundation and Lubin Center for Plant Biotechnology to Y.E.; from the German-Israeli Foundation for Scentific Research and Development to Y.E. and N.O.; and from the German-Israeli Project Cooperation to Y.E. and D.Z.",

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AU - Ori, Naomi

AU - Cohen, Aya Refael

AU - Etzioni, Adi

AU - Brand, Arnon

AU - Yanai, Osnat

AU - Shleizer, Sharona

AU - Menda, Naama

AU - Amsellem, Ziva

AU - Efroni, Idan

AU - Pekker, Irena

AU - Alvarez, John Paul

AU - Blum, Eyal

AU - Zamir, Dani

AU - Eshed, Yuval

N1 - Funding Information: We wish to thank E. Lifschitz5 (Technion), the C.M. Rick Tomato Genetics Resource Center and Clemson University Genomics Institute for materials, Navot Ori for unearthing la-6, and R. Fluhr, E. Hornstein, Z. Lippman, D. Weigel, S. Hake, J. Bowman and members of our laboratories for discussions and criticism. The work was supported by grants from the US-Israel Binational Agricultural Research and Development Fund, the European Union (MechPlant project) and the Israel Science Foundation (ISF) to N.O.; from ISF, MINERVA, The US-Israel Binational Science Foundation and Lubin Center for Plant Biotechnology to Y.E.; from the German-Israeli Foundation for Scentific Research and Development to Y.E. and N.O.; and from the German-Israeli Project Cooperation to Y.E. and D.Z.

PY - 2007/6

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N2 - Plant leaves show pronounced plasticity of size and form. In the classical, partially dominant mutation Lanceolate (La), the large compound leaves of tomato (Solanum lycopersicum) are converted into small simple ones. We show that LA encodes a transcription factor from the TCP family containing an miR319-binding site. Five independent La isolates are gain-of-function alleles that result from point mutations within the miR319-binding site and confer partial resistance of the La transcripts to microRNA (miRNA)-directed inhibition. The reduced sensitivity to miRNA regulation leads to elevated LA expression in very young La leaf primordia and to precocious differentiation of leaf margins. In contrast, downregulation of several LA-like genes using ectopic expression of miR319 resulted in larger leaflets and continuous growth of leaf margins. Our results imply that regulation of LA by miR319 defines a flexible window of morphogenetic competence along the developing leaf margin that is required for leaf elaboration.

AB - Plant leaves show pronounced plasticity of size and form. In the classical, partially dominant mutation Lanceolate (La), the large compound leaves of tomato (Solanum lycopersicum) are converted into small simple ones. We show that LA encodes a transcription factor from the TCP family containing an miR319-binding site. Five independent La isolates are gain-of-function alleles that result from point mutations within the miR319-binding site and confer partial resistance of the La transcripts to microRNA (miRNA)-directed inhibition. The reduced sensitivity to miRNA regulation leads to elevated LA expression in very young La leaf primordia and to precocious differentiation of leaf margins. In contrast, downregulation of several LA-like genes using ectopic expression of miR319 resulted in larger leaflets and continuous growth of leaf margins. Our results imply that regulation of LA by miR319 defines a flexible window of morphogenetic competence along the developing leaf margin that is required for leaf elaboration.

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Regulation of LANCEOLATE by miR319 is required for compound-leaf development in tomato

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