Regulation of splicing: The importance of being translatable

Elana Miriami, Ruth Sperling, Joseph Sperling*, Uzi Motro

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

16 Scopus citations

Abstract

RNA sequences that conform to the consensus sequence of 5′ splice sites but are not used for splicing occur frequently in protein coding genes. Mutational analyses have shown that suppression of splicing at such latent sites may be dictated by the necessity to maintain an open reading frame in the mRNA. Here we show that stop codon frequency in introns having latent 5′ splice sites is significantly greater than that of introns lacking such sites and significantly greater than the expected occurrence by chance alone. Both observations suggest the occurrence of a general mechanism that recognizes the mRNA reading frame in the context of pre-mRNA.

Original languageEnglish
Pages (from-to)1-4
Number of pages4
JournalRNA
Volume10
Issue number1
DOIs
StatePublished - Jan 2004

Keywords

  • Latent 5′ splice sites
  • Splice site selection
  • Stop codons
  • Suppression of splicing (SOS)
  • Translatability

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