Regulation of the establishment of repressor synthesis in bacteriophage λ

Amos B. Oppenheim*, Gamal Mahajna, Simi Koby, Shoshy Altuvia

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The bacteriophage λ cII and cIII gene products positively control the cI gene. In an attempt to define the site of action of cII and cIII, we constructed λ phages in which the lacZ gene is fused to the repressor establishment promoter PE. The cII and cIII genes were supplied by specially constructed plasmids. A DNA sequence extending from the middle of the cro gene to 58 base-pairs upstream of the PE transcription start site responds to cII and cIII. Since this fragment does not contain the oop region, we conclude that this region is dispensable for the positive regulation of cI. Neither PM nor PO are stimulated by cII and cIII, nor do they mediate the response of PE to these regulators. The experiments describe a simple in vivo assay system for the analysis of cII cIII control.

Original languageAmerican English
Pages (from-to)121-132
Number of pages12
JournalJournal of Molecular Biology
Volume155
Issue number2
DOIs
StatePublished - 25 Feb 1982

Bibliographical note

Funding Information:
We thauk Drs B. Meyer arld M. Ptashne for providing us with phage straius, aud Drs M. Roseuberg aud H. Shimatake for providiug plasmids ~1206 aud ~1314. We thank Drs M. Rosenberg arid M. Ptashue for providiug us with their results before publication. We thauk Mrs *Judy Srhniezer aud Mrs Diua Teff for help at various stages of this work. This work was supported iu part by a grant from the Uuitrd States-Israel Biuational Science Foundation arid the E.D. Bergman Science Foundation.

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