Relation between free fatty acid and acyl-CoA concentrations in rat brain following decapitation

Joseph Deutsch, S. I. Rapoport, A. David Purdon*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

70 Scopus citations

Abstract

To ascertain effects of total ischemia on brain phospholipid metabolism, anesthetized rats were decapitated and unesterified fatty acids and long chain acyl-CoA concentrations were analyzed in brain after 3 or 15 min. Control brain was taken from rats that were microwaved. Fatty acids were quantitated by extraction, thin layer chromatography and gas chromatography. Long-chain acylCoAs were quantitated by solubilization, solid phase extraction with an oligonucleotide purification cartridge and HPLC. Unesterified fatty acid concentrations increased significantly after decapitation, most dramatically for arachidonic acid (76 fold at 15 rain) followed by docosahexaenoic acid. Of the acyl-CoA molecular species only the concentration of arachidonoyl-CoA was increased at 3 rain and 15 rain after decapitation, by 3-4 fold compared with microwaved brain. The concentration of docosahexaenoyl-CoA fell whereas concentrations of the other acyl-CoAs were unchanged. The increase in arachidonoyl-CoA after decapitation indicates that reincorporation of arachidonic acid into membrane phospholipids is possible during ischemia, likely at the expense of docosahexaenoic acid.

Original languageEnglish
Pages (from-to)759-765
Number of pages7
JournalNeurochemical Research
Volume22
Issue number7
DOIs
StatePublished - 1997

Keywords

  • Acyl- CoA
  • Arachidonic acid
  • Brain
  • Docosahexaenoic acid
  • Fatty acids
  • Ischemia
  • Phospholipid
  • Rats
  • Stroke

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