TY - JOUR
T1 - Relationship between seawater pollution and qualitative changes in the extracted proteins from mussels Mytilus galloprovincialis
AU - Gorinstein, Shela
AU - Moncheva, Snejana
AU - Toledo, Fernando
AU - Arancibia-Avila, Patricia
AU - Trakhtenberg, Simon
AU - Gorinstein, Arkadi
AU - Goshev, Ivan
AU - Namiesnik, Jacek
PY - 2006/7/1
Y1 - 2006/7/1
N2 - The aim of this study was to find a reliable biomarker of seawater pollution. For this purpose the contents of Zn and Cu, proteins and antioxidant activity in mussels Mytilus galloprovincialis collected from polluted and non-polluted sites of the Bulgarian Black Sea coast were compared. To determine the above-mentioned indices atomic spectroscopy, Fourier Transform Infrared (FT-IR) spectroscopy, fluorescence, differential scanning calorimetry (DSC), and two antioxidant tests were used. It was found that the amounts of Zn and Cu were significantly higher in the mussel proteins from the polluted than from the non-polluted sites (P < 0.05). FT-IR spectroscopy and fluorescence revealed specific qualitative changes in secondary and tertiary structures in mussel proteins in the samples from polluted sites. The thermodynamic properties of proteins and the changes upon denaturation were correlated with the secondary structure of proteins and disappearance of α-helix. Purified protein scavenging activity against 2, 2′-azinobis (3-ethylbenzothiazoline-6-sulfonate) radical cation (ABTS•+) was significantly higher in mussel samples from polluted than from non-polluted sites. Therefore, the changes in Zn and Cu concentration, in protein's secondary and tertiary structures and antioxidant activity in mussels M. galloprovincialis from polluted sites can be a reliable biomarker of the level of the seawater pollution.
AB - The aim of this study was to find a reliable biomarker of seawater pollution. For this purpose the contents of Zn and Cu, proteins and antioxidant activity in mussels Mytilus galloprovincialis collected from polluted and non-polluted sites of the Bulgarian Black Sea coast were compared. To determine the above-mentioned indices atomic spectroscopy, Fourier Transform Infrared (FT-IR) spectroscopy, fluorescence, differential scanning calorimetry (DSC), and two antioxidant tests were used. It was found that the amounts of Zn and Cu were significantly higher in the mussel proteins from the polluted than from the non-polluted sites (P < 0.05). FT-IR spectroscopy and fluorescence revealed specific qualitative changes in secondary and tertiary structures in mussel proteins in the samples from polluted sites. The thermodynamic properties of proteins and the changes upon denaturation were correlated with the secondary structure of proteins and disappearance of α-helix. Purified protein scavenging activity against 2, 2′-azinobis (3-ethylbenzothiazoline-6-sulfonate) radical cation (ABTS•+) was significantly higher in mussel samples from polluted than from non-polluted sites. Therefore, the changes in Zn and Cu concentration, in protein's secondary and tertiary structures and antioxidant activity in mussels M. galloprovincialis from polluted sites can be a reliable biomarker of the level of the seawater pollution.
KW - Antioxidants activity
KW - Metals
KW - Mytilus galloprovincialis
KW - Pollution
KW - Proteins
KW - Structural changes
UR - http://www.scopus.com/inward/record.url?scp=33646837710&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2005.06.013
DO - 10.1016/j.scitotenv.2005.06.013
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C2 - 16198394
AN - SCOPUS:33646837710
SN - 0048-9697
VL - 364
SP - 251
EP - 259
JO - Science of the Total Environment
JF - Science of the Total Environment
IS - 1-3
ER -