Reliability of human cortical organoid generation

Se Jin Yoon; Lubayna S. Elahi; Anca M. Pașca; Rebecca M. Marton; Aaron Gordon; Omer Revah; Yuki Miura; Elisabeth M. Walczak; Gwendolyn M. Holdgate; H. Christina Fan; John R. Huguenard; Daniel H. Geschwind; Sergiu P. Pașca

doi:10.1038/s41592-018-0255-0

Reliability of human cortical organoid generation

Se Jin Yoon
, Lubayna S. Elahi
, Anca M. Pașca
, Rebecca M. Marton
, Aaron Gordon
, Omer Revah
, Yuki Miura
, Elisabeth M. Walczak
, Gwendolyn M. Holdgate
, H. Christina Fan
, John R. Huguenard
, Daniel H. Geschwind
, Sergiu P. Pașca^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

359 Scopus citations

Abstract

The differentiation of pluripotent stem cells in three-dimensional cultures can recapitulate key aspects of brain development, but protocols are prone to variable results. Here we differentiated multiple human pluripotent stem cell lines for over 100 d using our previously developed approach to generate brain-region-specific organoids called cortical spheroids and, using several assays, found that spheroid generation was highly reliable and consistent. We anticipate the use of this approach for large-scale differentiation experiments and disease modeling.

Original language	English
Pages (from-to)	75-78
Number of pages	4
Journal	Nature Methods
Volume	16
Issue number	1
DOIs	https://doi.org/10.1038/s41592-018-0255-0
State	Published - 1 Jan 2019
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2018, The Author(s), under exclusive licence to Springer Nature America, Inc.

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title = "Reliability of human cortical organoid generation",

abstract = "The differentiation of pluripotent stem cells in three-dimensional cultures can recapitulate key aspects of brain development, but protocols are prone to variable results. Here we differentiated multiple human pluripotent stem cell lines for over 100 d using our previously developed approach to generate brain-region-specific organoids called cortical spheroids and, using several assays, found that spheroid generation was highly reliable and consistent. We anticipate the use of this approach for large-scale differentiation experiments and disease modeling.",

author = "Yoon, \{Se Jin\} and Elahi, \{Lubayna S.\} and Pașca, \{Anca M.\} and Marton, \{Rebecca M.\} and Aaron Gordon and Omer Revah and Yuki Miura and Walczak, \{Elisabeth M.\} and Holdgate, \{Gwendolyn M.\} and Fan, \{H. Christina\} and Huguenard, \{John R.\} and Geschwind, \{Daniel H.\} and Pașca, \{Sergiu P.\}",

note = "Publisher Copyright: {\textcopyright} 2018, The Author(s), under exclusive licence to Springer Nature America, Inc.",

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doi = "10.1038/s41592-018-0255-0",

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AU - Pașca, Anca M.

AU - Marton, Rebecca M.

AU - Gordon, Aaron

AU - Revah, Omer

AU - Miura, Yuki

AU - Walczak, Elisabeth M.

AU - Holdgate, Gwendolyn M.

AU - Fan, H. Christina

AU - Huguenard, John R.

AU - Geschwind, Daniel H.

AU - Pașca, Sergiu P.

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Reliability of human cortical organoid generation

Abstract

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