Reliable determination of transposon insertion site in prokaryotes by direct sequencing

Udi Qimron; Neta Madar; Rivi Ascarelli-Goell; Maya Elgrably-Weiss; Shoshy Altuvia; Angel Porgador

doi:10.1016/S0167-7012(03)00033-2

Reliable determination of transposon insertion site in prokaryotes by direct sequencing

Udi Qimron, Neta Madar, Rivi Ascarelli-Goell, Maya Elgrably-Weiss, Shoshy Altuvia, Angel Porgador^*

^*Corresponding author for this work

Department of Microbiology and Molecular Genetics

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

We developed a method to identify the insertion sites of transposons in the chromosome of Salmonella using one step only. In this method, the Salmonella's genomic DNA is directly sequenced using a transposon internal primer. Reliable direct sequencing was achieved using high purity genomic DNA and an improved protocol for automated sequence machine. This note is intended to promote the use of direct sequencing, which we found to be reliable, efficient and inexpensive as compared to the other currently used methods.

Original language	English
Pages (from-to)	137-140
Number of pages	4
Journal	Journal of Microbiological Methods
Volume	54
Issue number	1
DOIs	https://doi.org/10.1016/S0167-7012(03)00033-2
State	Published - 1 Jul 2003

Bibliographical note

Funding Information:
This study was supported by grants from the USA–Israel Binational Science Foundation, Israel Cancer Research Foundation and Israel Cancer Association. This work eas also supported by the Cooperation Program of the Deutsches Krebsforschungszentrum (DKFZ) and Israel's Ministry of Science (MOS). U.Q. was supported by the Kreitman Foundation in BGU.

Keywords

Direct genomic sequencing
Transposon
Transposon insertion site

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10.1016/S0167-7012(03)00033-2

Cite this

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abstract = "We developed a method to identify the insertion sites of transposons in the chromosome of Salmonella using one step only. In this method, the Salmonella's genomic DNA is directly sequenced using a transposon internal primer. Reliable direct sequencing was achieved using high purity genomic DNA and an improved protocol for automated sequence machine. This note is intended to promote the use of direct sequencing, which we found to be reliable, efficient and inexpensive as compared to the other currently used methods.",

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AU - Elgrably-Weiss, Maya

AU - Altuvia, Shoshy

AU - Porgador, Angel

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KW - Direct genomic sequencing

KW - Transposon

KW - Transposon insertion site

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Reliable determination of transposon insertion site in prokaryotes by direct sequencing

Abstract

Bibliographical note

Keywords

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