Abstract
We developed a method to identify the insertion sites of transposons in the chromosome of Salmonella using one step only. In this method, the Salmonella's genomic DNA is directly sequenced using a transposon internal primer. Reliable direct sequencing was achieved using high purity genomic DNA and an improved protocol for automated sequence machine. This note is intended to promote the use of direct sequencing, which we found to be reliable, efficient and inexpensive as compared to the other currently used methods.
Original language | English |
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Pages (from-to) | 137-140 |
Number of pages | 4 |
Journal | Journal of Microbiological Methods |
Volume | 54 |
Issue number | 1 |
DOIs | |
State | Published - 1 Jul 2003 |
Bibliographical note
Funding Information:This study was supported by grants from the USA–Israel Binational Science Foundation, Israel Cancer Research Foundation and Israel Cancer Association. This work eas also supported by the Cooperation Program of the Deutsches Krebsforschungszentrum (DKFZ) and Israel's Ministry of Science (MOS). U.Q. was supported by the Kreitman Foundation in BGU.
Keywords
- Direct genomic sequencing
- Transposon
- Transposon insertion site