Reliable determination of transposon insertion site in prokaryotes by direct sequencing

Udi Qimron, Neta Madar, Rivi Ascarelli-Goell, Maya Elgrably-Weiss, Shoshy Altuvia, Angel Porgador*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

We developed a method to identify the insertion sites of transposons in the chromosome of Salmonella using one step only. In this method, the Salmonella's genomic DNA is directly sequenced using a transposon internal primer. Reliable direct sequencing was achieved using high purity genomic DNA and an improved protocol for automated sequence machine. This note is intended to promote the use of direct sequencing, which we found to be reliable, efficient and inexpensive as compared to the other currently used methods.

Original languageAmerican English
Pages (from-to)137-140
Number of pages4
JournalJournal of Microbiological Methods
Volume54
Issue number1
DOIs
StatePublished - 1 Jul 2003

Bibliographical note

Funding Information:
This study was supported by grants from the USA–Israel Binational Science Foundation, Israel Cancer Research Foundation and Israel Cancer Association. This work eas also supported by the Cooperation Program of the Deutsches Krebsforschungszentrum (DKFZ) and Israel's Ministry of Science (MOS). U.Q. was supported by the Kreitman Foundation in BGU.

Keywords

  • Direct genomic sequencing
  • Transposon
  • Transposon insertion site

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