Relief of cytotoxicity and enhancement of interferon inducer activity of double-stranded RNA by eukaryotic initiation factor 2

Ronit Harary; Rivkah Gonsky; Daliah Itamar; Raymond Kaempfer

doi:10.1016/0042-6822(90)90103-X

Relief of cytotoxicity and enhancement of interferon inducer activity of double-stranded RNA by eukaryotic initiation factor 2

Ronit Harary
, Rivkah Gonsky
, Daliah Itamar
, Raymond Kaempfer^*

^*Corresponding author for this work

Institute for Medical Research Israel-Canada (IMRIC)

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Double-stranded RNA (dsRNA) is a powerful interferon inducer but also possesses toxic properties. dsRNA was shown previously to inhibit translation by causing the inactivation of eukaryotic initiation factor 2 (eIF-2) and to bind tightly to this protein. The cytoxicity of dsRNA was analyzed simultaneously with the induction of interferon in murine fibroblast cultures. Incubation of dsRNA with eIF-2 leads to a significant reduction in toxicity, concomitant with a marked stimulation of interferon induction. The enhancement of dsRNA-dependent interferon induction by eIF-2 is sensitive to a monoclonal antibody directed against the β of eIF-2. When injected in combination with dsRNA, elF-2 potentiates the dsRNA-dependent survival of mice infected with a lethal dose of Mango virus.

Original language	English
Pages (from-to)	494-503
Number of pages	10
Journal	Virology
Volume	174
Issue number	2
DOIs	https://doi.org/10.1016/0042-6822(90)90103-X
State	Published - Feb 1990

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title = "Relief of cytotoxicity and enhancement of interferon inducer activity of double-stranded RNA by eukaryotic initiation factor 2",

abstract = "Double-stranded RNA (dsRNA) is a powerful interferon inducer but also possesses toxic properties. dsRNA was shown previously to inhibit translation by causing the inactivation of eukaryotic initiation factor 2 (eIF-2) and to bind tightly to this protein. The cytoxicity of dsRNA was analyzed simultaneously with the induction of interferon in murine fibroblast cultures. Incubation of dsRNA with eIF-2 leads to a significant reduction in toxicity, concomitant with a marked stimulation of interferon induction. The enhancement of dsRNA-dependent interferon induction by eIF-2 is sensitive to a monoclonal antibody directed against the β of eIF-2. When injected in combination with dsRNA, elF-2 potentiates the dsRNA-dependent survival of mice infected with a lethal dose of Mango virus.",

author = "Ronit Harary and Rivkah Gonsky and Daliah Itamar and Raymond Kaempfer",

year = "1990",

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doi = "10.1016/0042-6822(90)90103-X",

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AU - Harary, Ronit

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AU - Itamar, Daliah

AU - Kaempfer, Raymond

PY - 1990/2

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AB - Double-stranded RNA (dsRNA) is a powerful interferon inducer but also possesses toxic properties. dsRNA was shown previously to inhibit translation by causing the inactivation of eukaryotic initiation factor 2 (eIF-2) and to bind tightly to this protein. The cytoxicity of dsRNA was analyzed simultaneously with the induction of interferon in murine fibroblast cultures. Incubation of dsRNA with eIF-2 leads to a significant reduction in toxicity, concomitant with a marked stimulation of interferon induction. The enhancement of dsRNA-dependent interferon induction by eIF-2 is sensitive to a monoclonal antibody directed against the β of eIF-2. When injected in combination with dsRNA, elF-2 potentiates the dsRNA-dependent survival of mice infected with a lethal dose of Mango virus.

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Relief of cytotoxicity and enhancement of interferon inducer activity of double-stranded RNA by eukaryotic initiation factor 2

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