Replicative intermediates of herpes simplex virus DNA

Joseph Shlomai; Adam Friedmann; Yechiel Becker

doi:10.1016/0042-6822(76)90493-1

Replicative intermediates of herpes simplex virus DNA

Joseph Shlomai^*, Adam Friedmann, Yechiel Becker

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

The DNA synthesized in herpes simplex virus (HSV)-infected nuclei in vivo was analyzed by chromatography on benzoylated naphthoylated DEAE-cellulose columns. Viral-DNA molecules that elute with caffeine contain single-stranded DNA sequences sensitive to a micrococcal endonuclease that cleaves single-stranded DNA only. These viral-DNA molecules behave kinetically as precursors to mature virion DNA, and their transition to the mature double-stranded form occurs within a period of 10 to 20 min. Electron microscopy revealed viral-DNA molecules at different stages of replication. The mechanism of HSV-DNA replication is discussed.

Original language	English
Pages (from-to)	647-659
Number of pages	13
Journal	Virology
Volume	69
Issue number	2
DOIs	https://doi.org/10.1016/0042-6822(76)90493-1
State	Published - Feb 1976

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title = "Replicative intermediates of herpes simplex virus DNA",

abstract = "The DNA synthesized in herpes simplex virus (HSV)-infected nuclei in vivo was analyzed by chromatography on benzoylated naphthoylated DEAE-cellulose columns. Viral-DNA molecules that elute with caffeine contain single-stranded DNA sequences sensitive to a micrococcal endonuclease that cleaves single-stranded DNA only. These viral-DNA molecules behave kinetically as precursors to mature virion DNA, and their transition to the mature double-stranded form occurs within a period of 10 to 20 min. Electron microscopy revealed viral-DNA molecules at different stages of replication. The mechanism of HSV-DNA replication is discussed.",

author = "Joseph Shlomai and Adam Friedmann and Yechiel Becker",

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doi = "10.1016/0042-6822(76)90493-1",

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AB - The DNA synthesized in herpes simplex virus (HSV)-infected nuclei in vivo was analyzed by chromatography on benzoylated naphthoylated DEAE-cellulose columns. Viral-DNA molecules that elute with caffeine contain single-stranded DNA sequences sensitive to a micrococcal endonuclease that cleaves single-stranded DNA only. These viral-DNA molecules behave kinetically as precursors to mature virion DNA, and their transition to the mature double-stranded form occurs within a period of 10 to 20 min. Electron microscopy revealed viral-DNA molecules at different stages of replication. The mechanism of HSV-DNA replication is discussed.

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Replicative intermediates of herpes simplex virus DNA

Abstract

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