Resistance of soybean vegetative storage proteins (S-VSPs) to proteolysis by rumen microorganisms

Dana Guenoune, Serge Landau, Rachel Amir, Hana Badani, Levana Devash, Shmuel Wolf, Shmuel Galili*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Soybean vegetative storage proteins (S-VSPs) are lysine-rich and, hence, are potentially of high nutritive value for high productive ruminants. Using S-VSPs from wild-type soybean and from transgenic tobacco plants expressing either one of the two S-VSPs subunits (S-VSPα or S-VSPβ) or both, we tested their stability in cow rumen fluid under in situ conditions, using SDS-polyacrylamide gel electrophoresis. Proteolysis and degradation pattern of S-VSPs from transgenic tobacco leaves occurred relatively fast compared with that of wild-type (WT) soybean plants. Comparing the two S-VSPs subunits expressed in transgenic plants, we found that S-VSPα was degraded much faster than S-VSPβ. The degradation pattern of S-VSPs in transgenic tobacco plants expressing both subunits resembled that of WT soybean. In contrast, the degradation pattern of transgenic tobacco plants expressing a single subunit was different. These finding suggest that the quaternary structure of S-VSPs may be an important factor determining their resistance to rumen degradation. Our results also suggest that the stability to rumen proteolysis of a given protein, when expressed in a transgenic plant, may not always be predictable and has to be verified.

Original languageAmerican English
Pages (from-to)2256-2260
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Issue number8
StatePublished - 10 Apr 2002


  • Rumen proteolysis
  • Soybean vegetative storage proteins
  • Tobacco
  • Transgenic plants


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