TY - JOUR
T1 - Resisting Bacteria and Attracting Cells
T2 - Spontaneous Formation of a Bifunctional Peptide-Based Coating by On-Surface Assembly Approach
AU - Yuran, Sivan
AU - Dolid, Alona
AU - Reches, Meital
N1 - Publisher Copyright:
© 2018 American Chemical Society.
PY - 2018/12/10
Y1 - 2018/12/10
N2 - Due to extension of life expectancy, millions of people suffer nowadays from bone and dental malfunctions that can only be treated by different types of implants. However, these implants tend to fail due to bacterial infection and lack of integration with the remaining tissue. Here, we demonstrate a new concept in which we use specifically designed peptides, in a "Lego-like" manner to endow multiple preprogrammed functions. We developed a bifunctional peptide-based coating that simultaneously rejects the adhesion of infecting bacteria and attracts cells that build the new connecting tissue. The peptide design contains fluorinated phenylalanine that mediates the self-assembly of the peptide into a coating that resists bacterial adhesion. It also includes an Arg-Gly-Asp (RGD) motif that attracts mammalian cells. The whole compound is attached to the surface using a third unit, the amino acid 3,4-dihydroxyphenylalanine (DOPA). This novel, yet very simple approach is significantly advantageous for practical use and synthesis. More importantly, this peptide design can serve as a general platform for generating functional coatings.
AB - Due to extension of life expectancy, millions of people suffer nowadays from bone and dental malfunctions that can only be treated by different types of implants. However, these implants tend to fail due to bacterial infection and lack of integration with the remaining tissue. Here, we demonstrate a new concept in which we use specifically designed peptides, in a "Lego-like" manner to endow multiple preprogrammed functions. We developed a bifunctional peptide-based coating that simultaneously rejects the adhesion of infecting bacteria and attracts cells that build the new connecting tissue. The peptide design contains fluorinated phenylalanine that mediates the self-assembly of the peptide into a coating that resists bacterial adhesion. It also includes an Arg-Gly-Asp (RGD) motif that attracts mammalian cells. The whole compound is attached to the surface using a third unit, the amino acid 3,4-dihydroxyphenylalanine (DOPA). This novel, yet very simple approach is significantly advantageous for practical use and synthesis. More importantly, this peptide design can serve as a general platform for generating functional coatings.
KW - biofilm
KW - cell adhesion
KW - implants
KW - peptides
KW - self-assembly
UR - http://www.scopus.com/inward/record.url?scp=85056453720&partnerID=8YFLogxK
U2 - 10.1021/acsbiomaterials.8b00885
DO - 10.1021/acsbiomaterials.8b00885
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AN - SCOPUS:85056453720
SN - 2373-9878
VL - 4
SP - 4051
EP - 4061
JO - ACS Biomaterials Science and Engineering
JF - ACS Biomaterials Science and Engineering
IS - 12
ER -