Resolution of the Hemolytic and the Hydrolytic Activities of Phospholipase‐C Preparation from Clostridium perfringens

Esther Sabban*, Yehudith Laster, Abraham Loyter

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Phospholipase‐C preparations from Clostridium perfringens can induce hemolysis of chicken erythrocytes in the presence of EDTA. Under these conditions hydrolysis of membrane phospholipids does not take place. When Ca2+ is added to the incubation media both hemolysis and hydrolysis of phospholipids occur. However, when Mn2+ or UO22+ is added, both the hemolysis and hydrolysis of membrane phospholipids are strongly inhibited. When phospholipase‐C (Cl. perfringens) preparations are heated at 56°C in the presence of Ca2+, the hemolytic activity is inhibited while the hydrolytic activity of the enzyme is retained. Such non‐hemolytic phospholipase‐C barely hydrolyse phospholipids from intact chicken red‐blood cells. However, it readily hydrolyses phospholipids from erythrocyte ghosts. Thus it appears that membrane phospholipids are unavailable to the enzyme unless they are exposed by hemolysis.

Original languageEnglish
Pages (from-to)373-380
Number of pages8
JournalEuropean Journal of Biochemistry
Volume28
Issue number3
DOIs
StatePublished - Jul 1972

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