Resonant Raman scattering from cytochrome c: Frequency dependence of the depolarization ratio

David W. Collins; D. B. Fitchen; Aaron Lewis

doi:10.1063/1.1679924

Resonant Raman scattering from cytochrome c: Frequency dependence of the depolarization ratio

David W. Collins^*, D. B. Fitchen, Aaron Lewis

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

65 Scopus citations

Abstract

The depolarization ratio has been measured for several peaks in the resonant Raman spectrum of horse heart ferrocytochrome c (reduced) using six argon and krypton ion laser lines near resonance with the β absorption band. The depolarization ratio is observed to be a strong function of laser frequency so that previous mode symmetry assignments for heme proteins based on data at a single frequency are unreliable. For the anomalously polarized peaks (p₁ > 3/4), the depolarization ratio increases to a maximum when the laser frequency is midway between the a and βabsorption bands. These data are compared with a theoretical expression for the dispersion derived using perturbation theory to treat mixing of the D_4h basis states. Quantitative estimates of the relative contributions of the different symmetry representatives are obtained.

Original language	English
Pages (from-to)	5714-5719
Number of pages	6
Journal	The Journal of Chemical Physics
Volume	59
Issue number	10
DOIs	https://doi.org/10.1063/1.1679924
State	Published - 1973
Externally published	Yes

Access to Document

10.1063/1.1679924

Cite this

@article{c9d0449cb48f46b493d915d8bdf6b3a0,

title = "Resonant Raman scattering from cytochrome c: Frequency dependence of the depolarization ratio",

abstract = "The depolarization ratio has been measured for several peaks in the resonant Raman spectrum of horse heart ferrocytochrome c (reduced) using six argon and krypton ion laser lines near resonance with the β absorption band. The depolarization ratio is observed to be a strong function of laser frequency so that previous mode symmetry assignments for heme proteins based on data at a single frequency are unreliable. For the anomalously polarized peaks (p1 > 3/4), the depolarization ratio increases to a maximum when the laser frequency is midway between the a and βabsorption bands. These data are compared with a theoretical expression for the dispersion derived using perturbation theory to treat mixing of the D4h basis states. Quantitative estimates of the relative contributions of the different symmetry representatives are obtained.",

author = "Collins, {David W.} and Fitchen, {D. B.} and Aaron Lewis",

year = "1973",

doi = "10.1063/1.1679924",

language = "אנגלית",

volume = "59",

pages = "5714--5719",

journal = "The Journal of Chemical Physics",

issn = "0021-9606",

publisher = "American Institute of Physics",

number = "10",

}

TY - JOUR

T1 - Resonant Raman scattering from cytochrome c

T2 - Frequency dependence of the depolarization ratio

AU - Collins, David W.

AU - Fitchen, D. B.

AU - Lewis, Aaron

PY - 1973

Y1 - 1973

N2 - The depolarization ratio has been measured for several peaks in the resonant Raman spectrum of horse heart ferrocytochrome c (reduced) using six argon and krypton ion laser lines near resonance with the β absorption band. The depolarization ratio is observed to be a strong function of laser frequency so that previous mode symmetry assignments for heme proteins based on data at a single frequency are unreliable. For the anomalously polarized peaks (p1 > 3/4), the depolarization ratio increases to a maximum when the laser frequency is midway between the a and βabsorption bands. These data are compared with a theoretical expression for the dispersion derived using perturbation theory to treat mixing of the D4h basis states. Quantitative estimates of the relative contributions of the different symmetry representatives are obtained.

AB - The depolarization ratio has been measured for several peaks in the resonant Raman spectrum of horse heart ferrocytochrome c (reduced) using six argon and krypton ion laser lines near resonance with the β absorption band. The depolarization ratio is observed to be a strong function of laser frequency so that previous mode symmetry assignments for heme proteins based on data at a single frequency are unreliable. For the anomalously polarized peaks (p1 > 3/4), the depolarization ratio increases to a maximum when the laser frequency is midway between the a and βabsorption bands. These data are compared with a theoretical expression for the dispersion derived using perturbation theory to treat mixing of the D4h basis states. Quantitative estimates of the relative contributions of the different symmetry representatives are obtained.

UR - http://www.scopus.com/inward/record.url?scp=0000747884&partnerID=8YFLogxK

U2 - 10.1063/1.1679924

DO - 10.1063/1.1679924

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

AN - SCOPUS:0000747884

SN - 0021-9606

VL - 59

SP - 5714

EP - 5719

JO - The Journal of Chemical Physics

JF - The Journal of Chemical Physics

IS - 10

ER -

Resonant Raman scattering from cytochrome c: Frequency dependence of the depolarization ratio

Abstract

Access to Document

Other files and links

Fingerprint

Cite this