Respiratory control in submitochondrial particles and Ca++ transport

A. Loyter; R. O. Christiansen; E. Racker

doi:10.1016/0006-291X(67)90478-0

Respiratory control in submitochondrial particles and Ca⁺⁺ transport

A. Loyter^*, R. O. Christiansen, E. Racker

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

Submitochondrial particles obtained from beef heart mitochondria by sonic disruption accumulated Ca⁺⁺ during DPNH oxidation but not during succinate oxidation. Rotenone or antimycin inhibited the accumulation of Ca⁺⁺. Oxidation of DPNH but not of succinate was stimulated 4 to 5 fold by Ca⁺⁺. Uncouplers such as carbonylcyanide phenylhydrazone did not stimulate the oxidation of either DPNH or succinate and did not prevent the accumulation of Ca⁺⁺. These findings could not be explained by a lack of energy production with succinate as substrate, since both DPNH and succinate catalyzed oxidative phosphorylation with P:O ratios of 1.0 and 0.6 respectively.

Original language	English
Pages (from-to)	450-456
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	29
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(67)90478-0
State	Published - 17 Nov 1967
Externally published	Yes

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title = "Respiratory control in submitochondrial particles and Ca++ transport",

abstract = "Submitochondrial particles obtained from beef heart mitochondria by sonic disruption accumulated Ca++ during DPNH oxidation but not during succinate oxidation. Rotenone or antimycin inhibited the accumulation of Ca++. Oxidation of DPNH but not of succinate was stimulated 4 to 5 fold by Ca++. Uncouplers such as carbonylcyanide phenylhydrazone did not stimulate the oxidation of either DPNH or succinate and did not prevent the accumulation of Ca++. These findings could not be explained by a lack of energy production with succinate as substrate, since both DPNH and succinate catalyzed oxidative phosphorylation with P:O ratios of 1.0 and 0.6 respectively.",

author = "A. Loyter and Christiansen, {R. O.} and E. Racker",

year = "1967",

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T1 - Respiratory control in submitochondrial particles and Ca++ transport

AU - Loyter, A.

AU - Christiansen, R. O.

AU - Racker, E.

PY - 1967/11/17

Y1 - 1967/11/17

N2 - Submitochondrial particles obtained from beef heart mitochondria by sonic disruption accumulated Ca++ during DPNH oxidation but not during succinate oxidation. Rotenone or antimycin inhibited the accumulation of Ca++. Oxidation of DPNH but not of succinate was stimulated 4 to 5 fold by Ca++. Uncouplers such as carbonylcyanide phenylhydrazone did not stimulate the oxidation of either DPNH or succinate and did not prevent the accumulation of Ca++. These findings could not be explained by a lack of energy production with succinate as substrate, since both DPNH and succinate catalyzed oxidative phosphorylation with P:O ratios of 1.0 and 0.6 respectively.

AB - Submitochondrial particles obtained from beef heart mitochondria by sonic disruption accumulated Ca++ during DPNH oxidation but not during succinate oxidation. Rotenone or antimycin inhibited the accumulation of Ca++. Oxidation of DPNH but not of succinate was stimulated 4 to 5 fold by Ca++. Uncouplers such as carbonylcyanide phenylhydrazone did not stimulate the oxidation of either DPNH or succinate and did not prevent the accumulation of Ca++. These findings could not be explained by a lack of energy production with succinate as substrate, since both DPNH and succinate catalyzed oxidative phosphorylation with P:O ratios of 1.0 and 0.6 respectively.

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Respiratory control in submitochondrial particles and Ca⁺⁺ transport

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