Restriction fragment length polymorphism among Israeli Holstein‐Friesian dairy bulls

J. S. BECKMANN; Y. KASHI; E. M. HALLERMAN; A. NAVE; M. SOLLER

doi:10.1111/j.1365-2052.1986.tb03185.x

Restriction fragment length polymorphism among Israeli Holstein‐Friesian dairy bulls

J. S. BECKMANN^*, Y. KASHI, E. M. HALLERMAN, A. NAVE, M. SOLLER

^*Corresponding author for this work

Alexander Silberman Institute of Life Sciences

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Summary Israeli Holstein‐Friesian dairy bulls were screened for restriction fragment length polymorphisms by hybridizing cloned DNA probes for bovine growth hormone, for chymosin, and for rat muscle beta‐actin to restriction endonuclease‐digested DNA immobilized on nitrocellulose filters. The population proved to be polymorphic at the growth hormone locus, with evidence consistent with the phenotypes being inherited in allelic fashion. A low level of polymorphism was also observed at one of the beta‐actin gene family loci. The chymosin locus was monomorphic with the restriction enzymes utilized. The results illustrate the power of restriction fragment length polymorphism methodology in visualizing genetic variability in dairy cattle populations. 1986 International Society for Animal Genetics

Original language	English
Pages (from-to)	25-38
Number of pages	14
Journal	Animal Genetics
Volume	17
Issue number	1
DOIs	https://doi.org/10.1111/j.1365-2052.1986.tb03185.x
State	Published - Feb 1986

Keywords

RFLPs
dairy cattle
genetic markers
polymorphism
restriction fragments

Access to Document

10.1111/j.1365-2052.1986.tb03185.x

Cite this

@article{1f55ec3ba5fa4eba99ffee58c36a2400,

title = "Restriction fragment length polymorphism among Israeli Holstein‐Friesian dairy bulls",

abstract = "Summary Israeli Holstein‐Friesian dairy bulls were screened for restriction fragment length polymorphisms by hybridizing cloned DNA probes for bovine growth hormone, for chymosin, and for rat muscle beta‐actin to restriction endonuclease‐digested DNA immobilized on nitrocellulose filters. The population proved to be polymorphic at the growth hormone locus, with evidence consistent with the phenotypes being inherited in allelic fashion. A low level of polymorphism was also observed at one of the beta‐actin gene family loci. The chymosin locus was monomorphic with the restriction enzymes utilized. The results illustrate the power of restriction fragment length polymorphism methodology in visualizing genetic variability in dairy cattle populations. 1986 International Society for Animal Genetics",

keywords = "RFLPs, dairy cattle, genetic markers, polymorphism, restriction fragments",

author = "BECKMANN, \{J. S.\} and Y. KASHI and HALLERMAN, \{E. M.\} and A. NAVE and M. SOLLER",

year = "1986",

month = feb,

doi = "10.1111/j.1365-2052.1986.tb03185.x",

language = "אנגלית",

volume = "17",

pages = "25--38",

journal = "Animal Genetics",

issn = "0268-9146",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "1",

}

TY - JOUR

T1 - Restriction fragment length polymorphism among Israeli Holstein‐Friesian dairy bulls

AU - BECKMANN, J. S.

AU - KASHI, Y.

AU - HALLERMAN, E. M.

AU - NAVE, A.

AU - SOLLER, M.

PY - 1986/2

Y1 - 1986/2

N2 - Summary Israeli Holstein‐Friesian dairy bulls were screened for restriction fragment length polymorphisms by hybridizing cloned DNA probes for bovine growth hormone, for chymosin, and for rat muscle beta‐actin to restriction endonuclease‐digested DNA immobilized on nitrocellulose filters. The population proved to be polymorphic at the growth hormone locus, with evidence consistent with the phenotypes being inherited in allelic fashion. A low level of polymorphism was also observed at one of the beta‐actin gene family loci. The chymosin locus was monomorphic with the restriction enzymes utilized. The results illustrate the power of restriction fragment length polymorphism methodology in visualizing genetic variability in dairy cattle populations. 1986 International Society for Animal Genetics

AB - Summary Israeli Holstein‐Friesian dairy bulls were screened for restriction fragment length polymorphisms by hybridizing cloned DNA probes for bovine growth hormone, for chymosin, and for rat muscle beta‐actin to restriction endonuclease‐digested DNA immobilized on nitrocellulose filters. The population proved to be polymorphic at the growth hormone locus, with evidence consistent with the phenotypes being inherited in allelic fashion. A low level of polymorphism was also observed at one of the beta‐actin gene family loci. The chymosin locus was monomorphic with the restriction enzymes utilized. The results illustrate the power of restriction fragment length polymorphism methodology in visualizing genetic variability in dairy cattle populations. 1986 International Society for Animal Genetics

KW - RFLPs

KW - dairy cattle

KW - genetic markers

KW - polymorphism

KW - restriction fragments

UR - https://www.scopus.com/pages/publications/0022518769

U2 - 10.1111/j.1365-2052.1986.tb03185.x

DO - 10.1111/j.1365-2052.1986.tb03185.x

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 3013050

AN - SCOPUS:0022518769

SN - 0268-9146

VL - 17

SP - 25

EP - 38

JO - Animal Genetics

JF - Animal Genetics

IS - 1

ER -

Restriction fragment length polymorphism among Israeli Holstein‐Friesian dairy bulls

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this