Restriction fragment length polymorphism analysis of major histocompatibility complex class IV (B‐G) genotypes in meat‐type chickens

Z. UNI; J. HILLEL; R. WAIMAN; A. CAHANER; D. E. HELLER

doi:10.1111/j.1365-2052.1992.tb00162.x

Restriction fragment length polymorphism analysis of major histocompatibility complex class IV (B‐G) genotypes in meat‐type chickens

Z. UNI^*, J. HILLEL, R. WAIMAN, A. CAHANER, D. E. HELLER

^*Corresponding author for this work

Department of Animal Sciences

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Summary. Restriction fragment length polymorphism (RFLP) was used as a molecular genotyping approach to characterize differences in major histocompatibility complex class IV genes in meat‐type chickens. A high level of polymorphism was observed following digestion with each of the two restriction endonucleases Pvu II and BglII. Examination of DNA from 54 chickens revealed 23 polymorphic fragments. Application of RFLP techniques in the analysis of family groups should make possible the determination of B‐G genotypes in the meat type chickens.

Original language	English
Pages (from-to)	379-384
Number of pages	6
Journal	Animal Genetics
Volume	23
Issue number	4
DOIs	https://doi.org/10.1111/j.1365-2052.1992.tb00162.x
State	Published - Aug 1992

Keywords

MHC class IV
RFLP
chicken

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10.1111/j.1365-2052.1992.tb00162.x

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title = "Restriction fragment length polymorphism analysis of major histocompatibility complex class IV (B‐G) genotypes in meat‐type chickens",

abstract = "Summary. Restriction fragment length polymorphism (RFLP) was used as a molecular genotyping approach to characterize differences in major histocompatibility complex class IV genes in meat‐type chickens. A high level of polymorphism was observed following digestion with each of the two restriction endonucleases Pvu II and BglII. Examination of DNA from 54 chickens revealed 23 polymorphic fragments. Application of RFLP techniques in the analysis of family groups should make possible the determination of B‐G genotypes in the meat type chickens.",

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year = "1992",

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doi = "10.1111/j.1365-2052.1992.tb00162.x",

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AU - UNI, Z.

AU - HILLEL, J.

AU - WAIMAN, R.

AU - CAHANER, A.

AU - HELLER, D. E.

PY - 1992/8

Y1 - 1992/8

N2 - Summary. Restriction fragment length polymorphism (RFLP) was used as a molecular genotyping approach to characterize differences in major histocompatibility complex class IV genes in meat‐type chickens. A high level of polymorphism was observed following digestion with each of the two restriction endonucleases Pvu II and BglII. Examination of DNA from 54 chickens revealed 23 polymorphic fragments. Application of RFLP techniques in the analysis of family groups should make possible the determination of B‐G genotypes in the meat type chickens.

AB - Summary. Restriction fragment length polymorphism (RFLP) was used as a molecular genotyping approach to characterize differences in major histocompatibility complex class IV genes in meat‐type chickens. A high level of polymorphism was observed following digestion with each of the two restriction endonucleases Pvu II and BglII. Examination of DNA from 54 chickens revealed 23 polymorphic fragments. Application of RFLP techniques in the analysis of family groups should make possible the determination of B‐G genotypes in the meat type chickens.

KW - MHC class IV

KW - RFLP

KW - chicken

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ER -

Restriction fragment length polymorphism analysis of major histocompatibility complex class IV (B‐G) genotypes in meat‐type chickens

Abstract

Keywords

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