Restriction fragment length polymorphisms of 16s rDNA and of whole rRNA genes (ribotyping) of Streptococcus iniae strains from the United States and Israel

Avi Eldar; Sara Lawhon; Paul F. Frelier; Liliana Assenta; Bruce R. Simpson; Patricia W. Varner; Herve Bercovier

doi:10.1016/S0378-1097(97)00153-5

Restriction fragment length polymorphisms of 16s rDNA and of whole rRNA genes (ribotyping) of Streptococcus iniae strains from the United States and Israel

Avi Eldar, Sara Lawhon, Paul F. Frelier, Liliana Assenta, Bruce R. Simpson, Patricia W. Varner, Herve Bercovier^*

^*Corresponding author for this work

Alexander Silberman Institute of Life Sciences

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Streptococcus iniae (junior synonym S. shiloi) isolated from tilapia and trout in Israel and in the United States were subtyped by restriction length polymorphism (RFLP) based on PCR amplified 16S rDNA and by ribotyping. 16S rDNA RFLP discriminated between S. iniae and other fish pathogens but not between S. iniae strains. HindIII and EcoRI ribotypes of S. iniae discriminated American from Israeli strains rejecting the possibility of an epidemiological link between S. iniae infections in the two countries. Israeli strains isolated from tilapia and trout could not be completely differentiated. The S. iniae ATCC 29178(T) (T=Type strain) strain, isolated from a freshwater dolphin belonged to a ribotype different from those of all the fish isolates.

Original language	English
Pages (from-to)	155-162
Number of pages	8
Journal	FEMS Microbiology Letters
Volume	151
Issue number	2
DOIs	https://doi.org/10.1016/S0378-1097(97)00153-5
State	Published - 15 Jun 1997

Keywords

16S rDNA
Epidemiology
Restriction length polymorphism
Ribotype
Streptococcus iniae

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10.1016/S0378-1097(97)00153-5

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title = "Restriction fragment length polymorphisms of 16s rDNA and of whole rRNA genes (ribotyping) of Streptococcus iniae strains from the United States and Israel",

abstract = "Streptococcus iniae (junior synonym S. shiloi) isolated from tilapia and trout in Israel and in the United States were subtyped by restriction length polymorphism (RFLP) based on PCR amplified 16S rDNA and by ribotyping. 16S rDNA RFLP discriminated between S. iniae and other fish pathogens but not between S. iniae strains. HindIII and EcoRI ribotypes of S. iniae discriminated American from Israeli strains rejecting the possibility of an epidemiological link between S. iniae infections in the two countries. Israeli strains isolated from tilapia and trout could not be completely differentiated. The S. iniae ATCC 29178(T) (T=Type strain) strain, isolated from a freshwater dolphin belonged to a ribotype different from those of all the fish isolates.",

keywords = "16S rDNA, Epidemiology, Restriction length polymorphism, Ribotype, Streptococcus iniae",

author = "Avi Eldar and Sara Lawhon and {F. Frelier}, Paul and Liliana Assenta and Simpson, {Bruce R.} and Varner, {Patricia W.} and Herve Bercovier",

year = "1997",

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doi = "10.1016/S0378-1097(97)00153-5",

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AU - Eldar, Avi

AU - Lawhon, Sara

AU - F. Frelier, Paul

AU - Assenta, Liliana

AU - Simpson, Bruce R.

AU - Varner, Patricia W.

AU - Bercovier, Herve

PY - 1997/6/15

Y1 - 1997/6/15

N2 - Streptococcus iniae (junior synonym S. shiloi) isolated from tilapia and trout in Israel and in the United States were subtyped by restriction length polymorphism (RFLP) based on PCR amplified 16S rDNA and by ribotyping. 16S rDNA RFLP discriminated between S. iniae and other fish pathogens but not between S. iniae strains. HindIII and EcoRI ribotypes of S. iniae discriminated American from Israeli strains rejecting the possibility of an epidemiological link between S. iniae infections in the two countries. Israeli strains isolated from tilapia and trout could not be completely differentiated. The S. iniae ATCC 29178(T) (T=Type strain) strain, isolated from a freshwater dolphin belonged to a ribotype different from those of all the fish isolates.

AB - Streptococcus iniae (junior synonym S. shiloi) isolated from tilapia and trout in Israel and in the United States were subtyped by restriction length polymorphism (RFLP) based on PCR amplified 16S rDNA and by ribotyping. 16S rDNA RFLP discriminated between S. iniae and other fish pathogens but not between S. iniae strains. HindIII and EcoRI ribotypes of S. iniae discriminated American from Israeli strains rejecting the possibility of an epidemiological link between S. iniae infections in the two countries. Israeli strains isolated from tilapia and trout could not be completely differentiated. The S. iniae ATCC 29178(T) (T=Type strain) strain, isolated from a freshwater dolphin belonged to a ribotype different from those of all the fish isolates.

KW - 16S rDNA

KW - Epidemiology

KW - Restriction length polymorphism

KW - Ribotype

KW - Streptococcus iniae

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Restriction fragment length polymorphisms of 16s rDNA and of whole rRNA genes (ribotyping) of Streptococcus iniae strains from the United States and Israel

Abstract

Keywords

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