Restriction map of poliovirus type 2 cDNA.

H. Kopecka; S. van der Werf; F. Bregegere; N. Kitamura; M. Dreano; P. G. Rothberg; E. Wimmer; P. Kourilsky; M. Girard

Restriction map of poliovirus type 2 cDNA.

H. Kopecka^*, S. van der Werf, F. Bregegere, N. Kitamura, M. Dreano, P. G. Rothberg, E. Wimmer, P. Kourilsky, M. Girard

^*Corresponding author for this work

Department of Biological Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

Poliovirus type 1 RNA was reverse-transcribed into c-DNA and inserted at the Pst I site of the plasmid vector pBR322 of E. coli. Resulting recombinant plasmids were analyzed by hybridization with RNase T1-resistant 32P-labeled oligonucleotides, and by restriction enzyme mapping. All of the genome was cloned in a series overlapping cDNA inserts, the longest of which was 3.2 kb. The restriction map of the poliovirus cDNA is presented.

Original language	English
Pages (from-to)	301-309
Number of pages	9
Journal	Developments in Biological Standardization
Volume	50
State	Published - 1981

Cite this

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abstract = "Poliovirus type 1 RNA was reverse-transcribed into c-DNA and inserted at the Pst I site of the plasmid vector pBR322 of E. coli. Resulting recombinant plasmids were analyzed by hybridization with RNase T1-resistant 32P-labeled oligonucleotides, and by restriction enzyme mapping. All of the genome was cloned in a series overlapping cDNA inserts, the longest of which was 3.2 kb. The restriction map of the poliovirus cDNA is presented.",

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AU - Kopecka, H.

AU - van der Werf, S.

AU - Bregegere, F.

AU - Kitamura, N.

AU - Dreano, M.

AU - Rothberg, P. G.

AU - Wimmer, E.

AU - Kourilsky, P.

AU - Girard, M.

PY - 1981

Y1 - 1981

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AB - Poliovirus type 1 RNA was reverse-transcribed into c-DNA and inserted at the Pst I site of the plasmid vector pBR322 of E. coli. Resulting recombinant plasmids were analyzed by hybridization with RNase T1-resistant 32P-labeled oligonucleotides, and by restriction enzyme mapping. All of the genome was cloned in a series overlapping cDNA inserts, the longest of which was 3.2 kb. The restriction map of the poliovirus cDNA is presented.

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SN - 0301-5149

VL - 50

SP - 301

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JO - Developments in Biological Standardization

JF - Developments in Biological Standardization

ER -

Restriction map of poliovirus type 2 cDNA.

Abstract

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