TY - JOUR
T1 - Retinoic acid enhances the effect of collagen on bone union, following induced non-union defect in guinea pig ulna
AU - Sela, J.
AU - Kaufman, D.
AU - Shoshan, S.
AU - Shani, J.
PY - 2000
Y1 - 2000
N2 - Objective and Design: The aim of the present study is to evaluate the involvement of retinoic acid and collagen in wound healing, by combining them in a therapeutic modality for treating a non-union bone defect in a guinea-pig ulnar-bone model. Methods: a 4-mm disc was excised from the guinea-pig's ulnar-bone, and the space formed between the two ulnar fragments was filled with either collagen solution, retinoic acid solution or a combination of both. The guinea-pigs were sacrificed 2 or 6 weeks later, and the defected ulnar bones were studied by X-ray, by histology and by computerized histomorphometry. Results: After 6 weeks, the long bone area fraction within the histological sections of the bone, was increased after treatment with this mixture by 180%, as compared to the untreated controls. The cartilage area in those sections was decreased by 44% after the combined treatment, as compared to increases of 133% and 182% following treatments with collagen alone. Conclusion: These findings demonstrate that addition of 500 IU of retinoic acid to collagen at a site of a bone defect, is superior to either agent in enhancing regeneration of new bone, achieving union across the defect and leading to its complete repair.
AB - Objective and Design: The aim of the present study is to evaluate the involvement of retinoic acid and collagen in wound healing, by combining them in a therapeutic modality for treating a non-union bone defect in a guinea-pig ulnar-bone model. Methods: a 4-mm disc was excised from the guinea-pig's ulnar-bone, and the space formed between the two ulnar fragments was filled with either collagen solution, retinoic acid solution or a combination of both. The guinea-pigs were sacrificed 2 or 6 weeks later, and the defected ulnar bones were studied by X-ray, by histology and by computerized histomorphometry. Results: After 6 weeks, the long bone area fraction within the histological sections of the bone, was increased after treatment with this mixture by 180%, as compared to the untreated controls. The cartilage area in those sections was decreased by 44% after the combined treatment, as compared to increases of 133% and 182% following treatments with collagen alone. Conclusion: These findings demonstrate that addition of 500 IU of retinoic acid to collagen at a site of a bone defect, is superior to either agent in enhancing regeneration of new bone, achieving union across the defect and leading to its complete repair.
KW - Bone repair
KW - Bone union
KW - Collagen
KW - Ossification
KW - Retinoic acid
UR - http://www.scopus.com/inward/record.url?scp=0034495245&partnerID=8YFLogxK
U2 - 10.1007/s000110050646
DO - 10.1007/s000110050646
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C2 - 11211918
AN - SCOPUS:0034495245
SN - 1023-3830
VL - 49
SP - 679
EP - 683
JO - Inflammation Research
JF - Inflammation Research
IS - 12
ER -