Retinoic acid inhibits the myristoylation of a membrane protein in HL-60 cells

M. Almagor*, J. Bar-Tana

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Incubation of nondifferentiated HL-60 cells with high specific activity myristic acid results in myristoylation of a 25 KD membrane protein. Myristoylation was inhibited by retinoic acid but not by DMSO, thus indicating that the nonmyristoylated state induced by retinoic acid is causal to rather than the result of the overall differentiation sequel induced. Similarly, incubation of HL-60 cells with high specific activity retinoic acid results in specific retinoylation of a membrane protein of similar molecular mass. The two acylated proteins could however be separated by 2-D gel electrophoresis, thus indicating that inhibition of myristoylation by retinoic acid could not be accounted for by competition between retinoic and myristic acids for the same acylated site.

Original languageEnglish
Pages (from-to)877-882
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume172
Issue number2
DOIs
StatePublished - 30 Oct 1990

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