TY - JOUR
T1 - Retinoic acid inhibits the myristoylation of a membrane protein in HL-60 cells
AU - Almagor, M.
AU - Bar-Tana, J.
PY - 1990/10/30
Y1 - 1990/10/30
N2 - Incubation of nondifferentiated HL-60 cells with high specific activity myristic acid results in myristoylation of a 25 KD membrane protein. Myristoylation was inhibited by retinoic acid but not by DMSO, thus indicating that the nonmyristoylated state induced by retinoic acid is causal to rather than the result of the overall differentiation sequel induced. Similarly, incubation of HL-60 cells with high specific activity retinoic acid results in specific retinoylation of a membrane protein of similar molecular mass. The two acylated proteins could however be separated by 2-D gel electrophoresis, thus indicating that inhibition of myristoylation by retinoic acid could not be accounted for by competition between retinoic and myristic acids for the same acylated site.
AB - Incubation of nondifferentiated HL-60 cells with high specific activity myristic acid results in myristoylation of a 25 KD membrane protein. Myristoylation was inhibited by retinoic acid but not by DMSO, thus indicating that the nonmyristoylated state induced by retinoic acid is causal to rather than the result of the overall differentiation sequel induced. Similarly, incubation of HL-60 cells with high specific activity retinoic acid results in specific retinoylation of a membrane protein of similar molecular mass. The two acylated proteins could however be separated by 2-D gel electrophoresis, thus indicating that inhibition of myristoylation by retinoic acid could not be accounted for by competition between retinoic and myristic acids for the same acylated site.
UR - http://www.scopus.com/inward/record.url?scp=0025026401&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(90)90757-E
DO - 10.1016/0006-291X(90)90757-E
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 2241975
AN - SCOPUS:0025026401
SN - 0006-291X
VL - 172
SP - 877
EP - 882
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -