Revealing the ligand binding site of NhaA Na+/H+ antiporter and its pH dependence

Michal Maes, Abraham Rimon, Lena Kozachkov-Magrisso, Assaf Friedler*, Etana Padan

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

Background: Cell pH and Na+ homeostasis requires Na +/H+ antiporters such as NhaA. Results: Mutational analysis and ITC measurements revealed the NhaA-Li+ binding site. Conclusion: Binding of Li+ to purified NhaA is enthalpy-driven, highly specific, and pH-dependent and involves a single binding site. Significance: The pH-dependent NhaA-ligand binding is an insight into the mechanism of activity of NhaA and possibly other antiporters.

Original languageAmerican English
Pages (from-to)38150-38157
Number of pages8
JournalJournal of Biological Chemistry
Volume287
Issue number45
DOIs
StatePublished - 2 Nov 2012

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