TY - JOUR
T1 - Reversible effects of chaotropic agents on the proton permeability of Escherichia coli membrane vesicles
AU - Patel, L.
AU - Schuldiner, S.
AU - Kaback, H. R.
PY - 1975
Y1 - 1975
N2 - Extraction of E. coli ML 308-225 membrane vesicles with chaotropic agents causes the vesicles to become specifically permeable to protons. As a result, the vesicles no longer generate a membrane potential, interior negative, and they do not catalyze respiration dependent lactose or proline transport. Treatment of the extracted vesicles with various carbodiimides decreases the permeability of the vesicle membrane to protons, causing them to regain their ability to generate a membrane potential. By this means, active transport is completely reactivated. Exposure of the vesicles to carbodiimides prior to extraction with chaotropic agents makes transport activity impervious to the effects of the chaotropes.
AB - Extraction of E. coli ML 308-225 membrane vesicles with chaotropic agents causes the vesicles to become specifically permeable to protons. As a result, the vesicles no longer generate a membrane potential, interior negative, and they do not catalyze respiration dependent lactose or proline transport. Treatment of the extracted vesicles with various carbodiimides decreases the permeability of the vesicle membrane to protons, causing them to regain their ability to generate a membrane potential. By this means, active transport is completely reactivated. Exposure of the vesicles to carbodiimides prior to extraction with chaotropic agents makes transport activity impervious to the effects of the chaotropes.
UR - http://www.scopus.com/inward/record.url?scp=0016760409&partnerID=8YFLogxK
U2 - 10.1073/pnas.72.9.3387
DO - 10.1073/pnas.72.9.3387
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C2 - 171661
AN - SCOPUS:0016760409
SN - 0027-8424
VL - 72
SP - 3387
EP - 3391
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 9
ER -