Reward for Bdellovibrio bacteriovorus for preying on a polyhydroxyalkanoate producer

Virginia Martínez, Edouard Jurkevitch, José Luis García, María Auxiliadora Prieto*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


Bdellovibrio bacteriovorusHD100 is an obligate predator that invades and grows within the periplasm of Gram-negative bacteria, including mcl-polyhydroxyalkanoate (PHA) producers such as Pseudomonas putida. We investigated the impact of prey PHA content on the predator fitness and the potential advantages for preying on a PHA producer. Using a new procedure to control P. putidaKT2442 cell size we demonstrated that the number of Bdellovibrio progeny depends on the prey biomass and not on the viable prey cell number or PHA content. The presence of mcl-PHA hydrolysed products in the culture supernatant after predation on P. putidaKT42Z, a PHA producing strain lacking PhaZ depolymerase, confirmed the ability of Bdellovibrio to degrade the prey's PHA. Predator motility was higher when growing on PHA accumulating prey. External addition of PHA polymer (latex suspension) to Bdellovibrio preying on the PHA minus mutant P. putidaKT42C1 restored predator movement, suggesting that PHA is a key prey component to sustain predator swimming speed. High velocities observed in Bdellovibrio preying on the PHA producing strain were correlated to high intracellular ATP levels of the predator. These effects brought Bdellovibrio fitness benefits as predation on PHA producers was more efficient than predation on non-producing bacteria.

Original languageAmerican English
Pages (from-to)1204-1215
Number of pages12
JournalEnvironmental Microbiology
Issue number4
StatePublished - Apr 2013


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