RNA-binding protein conserved in both microtubule- and microfilament- based RNA localization

Leora Havin, Anna Git, Zichrini Elisha, Froma Oberman, Karina Yaniv, Sigal Pressman Schwartz, Nancy Standart, Joel K. Yisraeli*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

183 Scopus citations


Vg1 mRNA translocation to the vegetal cortex of Xenopus oocytes requires intact microtubules, and a 3' UTR cis-acting element (termed VLE), which also mediates sequence-specific binding of several proteins. One protein, the 69- kD Vg1 RBP, associates Vg1 RNA to microtubules in vitro. Here we shown that Vg1 RBP-binding sites correlate with vegetal localization. Purification and cloning of Vg1 RBP revealed five RNA-binding motifs: four KH and one RRM domains. Surprisingly, Vg1 RBP is highly homologous to the zipcode binding protein implicated in the microfilament-mediated localization of β actin mRNA in fibroblasts. These data support Vg1 RBP's direct role in vegetal localization and suggest the existence of a general, evolutionarily conserved mechanism for mRNA targeting.

Original languageAmerican English
Pages (from-to)1593-1598
Number of pages6
JournalGenes and Development
Issue number11
StatePublished - 1 Jun 1998


  • KH domain
  • Microtubules
  • Oocytes
  • RNA localization
  • Vgl RBP
  • Xenopus


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