RNAase-sensitive DNA-dependent DNA polymerase from rat cells transformed by avian sarcoma virus

Moshe Kotler; J. Rosenberg; Bertold Fridlender

doi:10.1016/0167-4781(84)90127-1

RNAase-sensitive DNA-dependent DNA polymerase from rat cells transformed by avian sarcoma virus

Moshe Kotler^*, J. Rosenberg, Bertold Fridlender

^*Corresponding author for this work

Institute for Medical Research Israel-Canada (IMRIC)

Research output: Contribution to journal › Article › peer-review

Abstract

An RNAase-sensitive DNA polymerase from rat cells transformed by avian sarcoma virus has been characterized. The enzyme requires RNA for its activity, as shown by its sensitivity to RNAase with endogenous as well as exogenous DNA templates. This sensitivity is maintained after its purification by sucrose gradients and ion exchange columns. A molecular weight of about 100 000 has been estimated. This DNA polymerase requires high salt concentration for its activity, is resistant to high concentrations of phosphonoacetic acid (400 μg/ml), is partially inhibited by 5 mM N-ethylmaleimide, and is completely inhibited by 0.3 mM parahydroxymercuribenzoate.

Original language	English
Pages (from-to)	92-99
Number of pages	8
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	781
Issue number	1-2
DOIs	https://doi.org/10.1016/0167-4781(84)90127-1
State	Published - 24 Feb 1984

Keywords

(Rat fibroblast)
Avian sarcoma virus
DNA polymerase
RNAase sensitivity

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10.1016/0167-4781(84)90127-1

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abstract = "An RNAase-sensitive DNA polymerase from rat cells transformed by avian sarcoma virus has been characterized. The enzyme requires RNA for its activity, as shown by its sensitivity to RNAase with endogenous as well as exogenous DNA templates. This sensitivity is maintained after its purification by sucrose gradients and ion exchange columns. A molecular weight of about 100 000 has been estimated. This DNA polymerase requires high salt concentration for its activity, is resistant to high concentrations of phosphonoacetic acid (400 μg/ml), is partially inhibited by 5 mM N-ethylmaleimide, and is completely inhibited by 0.3 mM parahydroxymercuribenzoate.",

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AU - Fridlender, Bertold

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RNAase-sensitive DNA-dependent DNA polymerase from rat cells transformed by avian sarcoma virus

Abstract

Keywords

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