TY - JOUR
T1 - Role for heme in mammalian protein synthesis
T2 - activation of an initiation factor
AU - Raffel, C.
AU - Stein, S.
AU - Kaempfer, R.
PY - 1974
Y1 - 1974
N2 - Two nonerythropoietic tissues, brain and liver, contain an initiation factor that can overcome the block in initiation of protein synthesis seen in reticulocyte lysates when exogenous hemin is not present. Upon incubation of the brain factor with hemin and removal of free hemin by gel filtration, the factor activity is strongly stimulated. This stimulation shows a concentration dependence on hemin close to that seen for stimulation of protein synthesis in whole reticulocyte lysates. The data indicate that hemin mediates the formation of an active initiation factor complex from inactive, lower mol wt components.
AB - Two nonerythropoietic tissues, brain and liver, contain an initiation factor that can overcome the block in initiation of protein synthesis seen in reticulocyte lysates when exogenous hemin is not present. Upon incubation of the brain factor with hemin and removal of free hemin by gel filtration, the factor activity is strongly stimulated. This stimulation shows a concentration dependence on hemin close to that seen for stimulation of protein synthesis in whole reticulocyte lysates. The data indicate that hemin mediates the formation of an active initiation factor complex from inactive, lower mol wt components.
UR - http://www.scopus.com/inward/record.url?scp=0016301824&partnerID=8YFLogxK
U2 - 10.1073/pnas.71.10.4020
DO - 10.1073/pnas.71.10.4020
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C2 - 4530282
AN - SCOPUS:0016301824
SN - 0027-8424
VL - 71
SP - 4020
EP - 4024
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 10
ER -