Role for heme in mammalian protein synthesis: activation of an initiation factor

C. Raffel, S. Stein, R. Kaempfer

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Two nonerythropoietic tissues, brain and liver, contain an initiation factor that can overcome the block in initiation of protein synthesis seen in reticulocyte lysates when exogenous hemin is not present. Upon incubation of the brain factor with hemin and removal of free hemin by gel filtration, the factor activity is strongly stimulated. This stimulation shows a concentration dependence on hemin close to that seen for stimulation of protein synthesis in whole reticulocyte lysates. The data indicate that hemin mediates the formation of an active initiation factor complex from inactive, lower mol wt components.

Original languageEnglish
Pages (from-to)4020-4024
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume71
Issue number10
DOIs
StatePublished - 1974
Externally publishedYes

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