Role of Nudt2 in Anchorage-Independent Growth and Cell Migration of Human Melanoma

Sana’ Hidmi, Hovav Nechushtan, Ehud Razin*, Sagi Tshori

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Nudt2 encodes a diadenosine tetraphosphate (Ap4A) hydrolase that catalyzes the hydrolysis of Ap4A and is involved in the lysyl tRNA synthetase-Ap4A-Nudt2 (LysRS-Ap4A-Nudt2) signaling pathway. We have previously demonstrated that this pathway is active in non-small cell lung cancer. Nudt2 was shown to be involved in cell proliferation in breast cancer, making it an important target in cancer therapy. Currently, the function of Nudt2 in malignant melanoma has not been demonstrated. Therefore, we investigated the role played by Nudt2 in the growth of human melanoma. Our study showed that Nudt2 knockdown suppressed anchorage-independent growth of human melanoma cells in vitro. The in vivo effect of Nudt2 was determined by investigating the role played by Nudt2 knockdown on the ability of the cells to form tumors in a mice xenograft model. Nudt2 knockdown significantly suppressed tumor growth in this model. Moreover, overexpression of Nudt2 resulted in an increase in anchorage-independent growth of these cells, whereas Nudt2 knockdown decreased their migration. In addition, Nudt2 knockdown reduced vimentin expression. Vimentin is one of the mesenchymal markers that are involved in the epithelial mesenchymal transition (EMT) process. Thus, Nudt2 plays an important role in promoting anchorage-independent growth and cell migration in melanoma.

Original languageEnglish
Article number10513
JournalInternational Journal of Molecular Sciences
Volume24
Issue number13
DOIs
StatePublished - Jul 2023

Bibliographical note

Publisher Copyright:
© 2023 by the authors.

Keywords

  • anchorage-independent growth
  • cell migration
  • melanoma
  • Nudt2
  • xenograft model

Fingerprint

Dive into the research topics of 'Role of Nudt2 in Anchorage-Independent Growth and Cell Migration of Human Melanoma'. Together they form a unique fingerprint.

Cite this