Role of the polyadenylate segment in the translantion of globin messenger RNA in Xenopus oocytes

G. Huez; G. Marbaix; E. Hubert; M. Leclercq; U. Nudel; H. Soreq; R. Salomon; B. Lebleu; M. Revel; U. Z. Littauer

doi:10.1073/pnas.71.8.3143

Role of the polyadenylate segment in the translantion of globin messenger RNA in Xenopus oocytes

G. Huez, G. Marbaix, E. Hubert, M. Leclercq, U. Nudel, H. Soreq, R. Salomon, B. Lebleu, M. Revel, U. Z. Littauer

Alexander Silberman Institute of Life Sciences

Research output: Contribution to journal › Article › peer-review

119 Scopus citations

Abstract

The translations of native messenger RNA for rabbit globin and that of poly(A) free globin messenger RNA have been compared after injection into Xenopus oocytes. The initial rate of translation of poly(A) free mRNA is close to that found for intact mRNA. However, at longer incubation periods, the rate of globin synthesis with poly(A) free mRNA is considerably lower than with native mRNA. Similar differences in the template activity of the two mRNA preparations were found with a cell free extract of Krebs II ascites tumor. It is concluded that the presence of the 3' poly(A) rich sequence in mRNA is required to ensure high functional stability.

Original language	English
Pages (from-to)	3143-3146
Number of pages	4
Journal	Unknown Journal
Volume	71
Issue number	8
DOIs	https://doi.org/10.1073/pnas.71.8.3143
State	Published - 1974

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abstract = "The translations of native messenger RNA for rabbit globin and that of poly(A) free globin messenger RNA have been compared after injection into Xenopus oocytes. The initial rate of translation of poly(A) free mRNA is close to that found for intact mRNA. However, at longer incubation periods, the rate of globin synthesis with poly(A) free mRNA is considerably lower than with native mRNA. Similar differences in the template activity of the two mRNA preparations were found with a cell free extract of Krebs II ascites tumor. It is concluded that the presence of the 3' poly(A) rich sequence in mRNA is required to ensure high functional stability.",

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AU - Huez, G.

AU - Marbaix, G.

AU - Hubert, E.

AU - Leclercq, M.

AU - Nudel, U.

AU - Soreq, H.

AU - Salomon, R.

AU - Lebleu, B.

AU - Revel, M.

AU - Littauer, U. Z.

PY - 1974

Y1 - 1974

N2 - The translations of native messenger RNA for rabbit globin and that of poly(A) free globin messenger RNA have been compared after injection into Xenopus oocytes. The initial rate of translation of poly(A) free mRNA is close to that found for intact mRNA. However, at longer incubation periods, the rate of globin synthesis with poly(A) free mRNA is considerably lower than with native mRNA. Similar differences in the template activity of the two mRNA preparations were found with a cell free extract of Krebs II ascites tumor. It is concluded that the presence of the 3' poly(A) rich sequence in mRNA is required to ensure high functional stability.

AB - The translations of native messenger RNA for rabbit globin and that of poly(A) free globin messenger RNA have been compared after injection into Xenopus oocytes. The initial rate of translation of poly(A) free mRNA is close to that found for intact mRNA. However, at longer incubation periods, the rate of globin synthesis with poly(A) free mRNA is considerably lower than with native mRNA. Similar differences in the template activity of the two mRNA preparations were found with a cell free extract of Krebs II ascites tumor. It is concluded that the presence of the 3' poly(A) rich sequence in mRNA is required to ensure high functional stability.

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Role of the polyadenylate segment in the translantion of globin messenger RNA in Xenopus oocytes

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