Role of antioxidants in prevention of pyrimidine dimer formation in UVB irradiated human HaCaT keratinocytes

Malka Hochberg; Ron Kohen; Claes D. Enk

doi:10.1016/j.biopha.2006.04.008

Role of antioxidants in prevention of pyrimidine dimer formation in UVB irradiated human HaCaT keratinocytes

Malka Hochberg, Ron Kohen^*, Claes D. Enk

^*Corresponding author for this work

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

The objective of the present study was to study the role of reactive oxygen species (ROS) in UVB induced cyclobutane pyrimidine dimer (CPD) formation in human keratinocytes, and to examine the modulating activity of low molecular weight antioxidants. To demonstrate the involvement of ROS, we examined the protective capacity of α-tocopherol, tempamine, and diethyldithiocarbamate (DDC) on CPD formation in intact cells and naked DNA. HaCaT cells and naked DNA in water solution were irradiated with UVB in the presence of the antioxidants and CPD was determined by ELISA. We found that all three antioxidants provided protection against UVB induced CPD formation. The protection was observed in intact cells only and not in naked DNA. Since some of the tested antioxidants do not possess UV absorbing qualities, our findings suggest that in a cellular environment ROS play a role in CPD formation.

Original language	English
Pages (from-to)	233-237
Number of pages	5
Journal	Biomedicine and Pharmacotherapy
Volume	60
Issue number	5
DOIs	https://doi.org/10.1016/j.biopha.2006.04.008
State	Published - Jun 2006

Bibliographical note

Funding Information:
Supported by grants from The Israel Cancer Association through the donation from The Brown Foundation, Florida, (grant no. 20030004-B), The Dead Sea Research Center, Israel, grant no. 422(6)-2-M) and The Eva and Henry Fraenkel Memorial Foundation, Copenhagen, Denmark.

Keywords

Antioxidants
HaCaT keratinocytes
Pyrinidine dimers
UVB
α-Tocopherol

Access to Document

10.1016/j.biopha.2006.04.008

Cite this

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title = "Role of antioxidants in prevention of pyrimidine dimer formation in UVB irradiated human HaCaT keratinocytes",

abstract = "The objective of the present study was to study the role of reactive oxygen species (ROS) in UVB induced cyclobutane pyrimidine dimer (CPD) formation in human keratinocytes, and to examine the modulating activity of low molecular weight antioxidants. To demonstrate the involvement of ROS, we examined the protective capacity of α-tocopherol, tempamine, and diethyldithiocarbamate (DDC) on CPD formation in intact cells and naked DNA. HaCaT cells and naked DNA in water solution were irradiated with UVB in the presence of the antioxidants and CPD was determined by ELISA. We found that all three antioxidants provided protection against UVB induced CPD formation. The protection was observed in intact cells only and not in naked DNA. Since some of the tested antioxidants do not possess UV absorbing qualities, our findings suggest that in a cellular environment ROS play a role in CPD formation.",

keywords = "Antioxidants, HaCaT keratinocytes, Pyrinidine dimers, UVB, α-Tocopherol",

author = "Malka Hochberg and Ron Kohen and Enk, {Claes D.}",

note = "Funding Information: Supported by grants from The Israel Cancer Association through the donation from The Brown Foundation, Florida, (grant no. 20030004-B), The Dead Sea Research Center, Israel, grant no. 422(6)-2-M) and The Eva and Henry Fraenkel Memorial Foundation, Copenhagen, Denmark.",

year = "2006",

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AU - Kohen, Ron

AU - Enk, Claes D.

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N2 - The objective of the present study was to study the role of reactive oxygen species (ROS) in UVB induced cyclobutane pyrimidine dimer (CPD) formation in human keratinocytes, and to examine the modulating activity of low molecular weight antioxidants. To demonstrate the involvement of ROS, we examined the protective capacity of α-tocopherol, tempamine, and diethyldithiocarbamate (DDC) on CPD formation in intact cells and naked DNA. HaCaT cells and naked DNA in water solution were irradiated with UVB in the presence of the antioxidants and CPD was determined by ELISA. We found that all three antioxidants provided protection against UVB induced CPD formation. The protection was observed in intact cells only and not in naked DNA. Since some of the tested antioxidants do not possess UV absorbing qualities, our findings suggest that in a cellular environment ROS play a role in CPD formation.

AB - The objective of the present study was to study the role of reactive oxygen species (ROS) in UVB induced cyclobutane pyrimidine dimer (CPD) formation in human keratinocytes, and to examine the modulating activity of low molecular weight antioxidants. To demonstrate the involvement of ROS, we examined the protective capacity of α-tocopherol, tempamine, and diethyldithiocarbamate (DDC) on CPD formation in intact cells and naked DNA. HaCaT cells and naked DNA in water solution were irradiated with UVB in the presence of the antioxidants and CPD was determined by ELISA. We found that all three antioxidants provided protection against UVB induced CPD formation. The protection was observed in intact cells only and not in naked DNA. Since some of the tested antioxidants do not possess UV absorbing qualities, our findings suggest that in a cellular environment ROS play a role in CPD formation.

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