TY - JOUR
T1 - Root Regeneration Triggers an Embryo-like Sequence Guided by Hormonal Interactions
AU - Efroni, Idan
AU - Mello, Alison
AU - Nawy, Tal
AU - Ip, Pui Leng
AU - Rahni, Ramin
AU - Delrose, Nicholas
AU - Powers, Ashley
AU - Satija, Rahul
AU - Birnbaum, Kenneth D.
N1 - Publisher Copyright:
© 2016 Elsevier Inc.
PY - 2016/6/16
Y1 - 2016/6/16
N2 - Plant roots can regenerate after excision of their tip, including the stem cell niche. To determine which developmental program mediates such repair, we applied a combination of lineage tracing, single-cell RNA sequencing, and marker analysis to test different models of tissue reassembly. We show that multiple cell types can reconstitute stem cells, demonstrating the latent potential of untreated plant cells. The transcriptome of regenerating cells prior to stem cell activation resembles that of an embryonic root progenitor. Regeneration defects are more severe in embryonic than in adult root mutants. Furthermore, the signaling domains of the hormones auxin and cytokinin mirror their embryonic dynamics and manipulation of both hormones alters the position of new tissues and stem cell niche markers. Our findings suggest that plant root regeneration follows, on a larger scale, the developmental stages of embryonic patterning and is guided by spatial information provided by complementary hormone domains.
AB - Plant roots can regenerate after excision of their tip, including the stem cell niche. To determine which developmental program mediates such repair, we applied a combination of lineage tracing, single-cell RNA sequencing, and marker analysis to test different models of tissue reassembly. We show that multiple cell types can reconstitute stem cells, demonstrating the latent potential of untreated plant cells. The transcriptome of regenerating cells prior to stem cell activation resembles that of an embryonic root progenitor. Regeneration defects are more severe in embryonic than in adult root mutants. Furthermore, the signaling domains of the hormones auxin and cytokinin mirror their embryonic dynamics and manipulation of both hormones alters the position of new tissues and stem cell niche markers. Our findings suggest that plant root regeneration follows, on a larger scale, the developmental stages of embryonic patterning and is guided by spatial information provided by complementary hormone domains.
UR - http://www.scopus.com/inward/record.url?scp=84975275160&partnerID=8YFLogxK
U2 - 10.1016/j.cell.2016.04.046
DO - 10.1016/j.cell.2016.04.046
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C2 - 27212234
AN - SCOPUS:84975275160
SN - 0092-8674
VL - 165
SP - 1721
EP - 1733
JO - Cell
JF - Cell
IS - 7
ER -