Rose scent: Genomics approach to discovering novel floral fragrance-related genes

Inna Guterman, Moshe Shalit, Naama Menda, Dan Piestun, Mery Dafny-Yelin, Gil Shalev, Einat Bar, Olga Davydov, Mariana Ovadis, Michal Emanuel, Jihong Wang, Zach Adam, Eran Pichersky, Efraim Lewinsohn, Dani Zamir, Alexander Vainstein, David Weiss*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

268 Scopus citations

Abstract

For centuries, rose has been the most important crop in the floriculture industry; its economic importance also lies in the use of its petals as a source of natural fragrances. Here, we used genomics approaches to identify novel scent-related genes, using rose flowers from tetraploid scented and nonscented cultivars. An annotated petal EST database of ∼2100 unique genes from both cultivars was created, and DNA chips were prepared and used for expression analyses of selected clones. Detailed chemical analysis of volatile composition in the two cultivars, together with the identification of secondary metabolism-related genes whose expression coincides with scent production, led to the discovery of several novel flower scent-related candidate genes. The function of some of these genes, including a germacrene D synthase, was biochemically determined using an Escherichia coli expression system. This work demonstrates the advantages of using the high-throughput approaches of genomics to detail traits of interest expressed in a cultivar-specific manner in nonmodel plants.

Original languageAmerican English
Pages (from-to)2325-2338
Number of pages14
JournalPlant Cell
Volume14
Issue number10
DOIs
StatePublished - 1 Oct 2002

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