Rose scent: Genomics approach to discovering novel floral fragrance-related genes

Inna Guterman; Moshe Shalit; Naama Menda; Dan Piestun; Mery Dafny-Yelin; Gil Shalev; Einat Bar; Olga Davydov; Mariana Ovadis; Michal Emanuel; Jihong Wang; Zach Adam; Eran Pichersky; Efraim Lewinsohn; Dani Zamir; Alexander Vainstein; David Weiss

doi:10.1105/tpc.005207

Rose scent: Genomics approach to discovering novel floral fragrance-related genes

Inna Guterman, Moshe Shalit, Naama Menda, Dan Piestun, Mery Dafny-Yelin, Gil Shalev, Einat Bar, Olga Davydov, Mariana Ovadis, Michal Emanuel, Jihong Wang, Zach Adam, Eran Pichersky, Efraim Lewinsohn, Dani Zamir, Alexander Vainstein, David Weiss^*

^*Corresponding author for this work

Institute of Plant Sciences and Genetics in Agriculture

Research output: Contribution to journal › Article › peer-review

273 Scopus citations

Abstract

For centuries, rose has been the most important crop in the floriculture industry; its economic importance also lies in the use of its petals as a source of natural fragrances. Here, we used genomics approaches to identify novel scent-related genes, using rose flowers from tetraploid scented and nonscented cultivars. An annotated petal EST database of ∼2100 unique genes from both cultivars was created, and DNA chips were prepared and used for expression analyses of selected clones. Detailed chemical analysis of volatile composition in the two cultivars, together with the identification of secondary metabolism-related genes whose expression coincides with scent production, led to the discovery of several novel flower scent-related candidate genes. The function of some of these genes, including a germacrene D synthase, was biochemically determined using an Escherichia coli expression system. This work demonstrates the advantages of using the high-throughput approaches of genomics to detail traits of interest expressed in a cultivar-specific manner in nonmodel plants.

Original language	English
Pages (from-to)	2325-2338
Number of pages	14
Journal	Plant Cell
Volume	14
Issue number	10
DOIs	https://doi.org/10.1105/tpc.005207
State	Published - 1 Oct 2002

Access to Document

10.1105/tpc.005207

Cite this

Guterman, I., Shalit, M., Menda, N., Piestun, D., Dafny-Yelin, M., Shalev, G., Bar, E., Davydov, O., Ovadis, M., Emanuel, M., Wang, J., Adam, Z., Pichersky, E., Lewinsohn, E., Zamir, D., Vainstein, A., & Weiss, D. (2002). Rose scent: Genomics approach to discovering novel floral fragrance-related genes. Plant Cell, 14(10), 2325-2338. https://doi.org/10.1105/tpc.005207

@article{97c7a7ec1576408a8468358abe4f54ab,

title = "Rose scent: Genomics approach to discovering novel floral fragrance-related genes",

abstract = "For centuries, rose has been the most important crop in the floriculture industry; its economic importance also lies in the use of its petals as a source of natural fragrances. Here, we used genomics approaches to identify novel scent-related genes, using rose flowers from tetraploid scented and nonscented cultivars. An annotated petal EST database of ∼2100 unique genes from both cultivars was created, and DNA chips were prepared and used for expression analyses of selected clones. Detailed chemical analysis of volatile composition in the two cultivars, together with the identification of secondary metabolism-related genes whose expression coincides with scent production, led to the discovery of several novel flower scent-related candidate genes. The function of some of these genes, including a germacrene D synthase, was biochemically determined using an Escherichia coli expression system. This work demonstrates the advantages of using the high-throughput approaches of genomics to detail traits of interest expressed in a cultivar-specific manner in nonmodel plants.",

author = "Inna Guterman and Moshe Shalit and Naama Menda and Dan Piestun and Mery Dafny-Yelin and Gil Shalev and Einat Bar and Olga Davydov and Mariana Ovadis and Michal Emanuel and Jihong Wang and Zach Adam and Eran Pichersky and Efraim Lewinsohn and Dani Zamir and Alexander Vainstein and David Weiss",

year = "2002",

month = oct,

day = "1",

doi = "10.1105/tpc.005207",

language = "אנגלית",

volume = "14",

pages = "2325--2338",

journal = "Plant Cell",

issn = "1040-4651",

publisher = "American Society of Plant Biologists",

number = "10",

}

TY - JOUR

T1 - Rose scent

T2 - Genomics approach to discovering novel floral fragrance-related genes

AU - Guterman, Inna

AU - Shalit, Moshe

AU - Menda, Naama

AU - Piestun, Dan

AU - Dafny-Yelin, Mery

AU - Shalev, Gil

AU - Bar, Einat

AU - Davydov, Olga

AU - Ovadis, Mariana

AU - Emanuel, Michal

AU - Wang, Jihong

AU - Adam, Zach

AU - Pichersky, Eran

AU - Lewinsohn, Efraim

AU - Zamir, Dani

AU - Vainstein, Alexander

AU - Weiss, David

PY - 2002/10/1

Y1 - 2002/10/1

N2 - For centuries, rose has been the most important crop in the floriculture industry; its economic importance also lies in the use of its petals as a source of natural fragrances. Here, we used genomics approaches to identify novel scent-related genes, using rose flowers from tetraploid scented and nonscented cultivars. An annotated petal EST database of ∼2100 unique genes from both cultivars was created, and DNA chips were prepared and used for expression analyses of selected clones. Detailed chemical analysis of volatile composition in the two cultivars, together with the identification of secondary metabolism-related genes whose expression coincides with scent production, led to the discovery of several novel flower scent-related candidate genes. The function of some of these genes, including a germacrene D synthase, was biochemically determined using an Escherichia coli expression system. This work demonstrates the advantages of using the high-throughput approaches of genomics to detail traits of interest expressed in a cultivar-specific manner in nonmodel plants.

AB - For centuries, rose has been the most important crop in the floriculture industry; its economic importance also lies in the use of its petals as a source of natural fragrances. Here, we used genomics approaches to identify novel scent-related genes, using rose flowers from tetraploid scented and nonscented cultivars. An annotated petal EST database of ∼2100 unique genes from both cultivars was created, and DNA chips were prepared and used for expression analyses of selected clones. Detailed chemical analysis of volatile composition in the two cultivars, together with the identification of secondary metabolism-related genes whose expression coincides with scent production, led to the discovery of several novel flower scent-related candidate genes. The function of some of these genes, including a germacrene D synthase, was biochemically determined using an Escherichia coli expression system. This work demonstrates the advantages of using the high-throughput approaches of genomics to detail traits of interest expressed in a cultivar-specific manner in nonmodel plants.

UR - http://www.scopus.com/inward/record.url?scp=0036801491&partnerID=8YFLogxK

U2 - 10.1105/tpc.005207

DO - 10.1105/tpc.005207

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 12368489

AN - SCOPUS:0036801491

SN - 1040-4651

VL - 14

SP - 2325

EP - 2338

JO - Plant Cell

JF - Plant Cell

IS - 10

ER -

Rose scent: Genomics approach to discovering novel floral fragrance-related genes

Abstract

Access to Document

Other files and links

Fingerprint

Cite this