Routine dot-blot assay of multiple serum samples using a simple apparatus

Mariano Zalis; Charles L. Jaffe

doi:10.1016/0022-1759(87)90158-X

Routine dot-blot assay of multiple serum samples using a simple apparatus

Mariano Zalis, Charles L. Jaffe^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

This paper describes a simple method for carrying out dot-blot immunoassays simultaneously with a large number of different sera. No dot-blot manifold is necessary, only a modified 96-well flat-bottom microtiter plate. This apparatus is re-usable, inexpensive and especially suited for field testing of patient sera in serodiagnostic assays. One application of this technique, to the serodiagnosis of visceral leishmaniasis on a pure parasite protein, is demonstrated.

Original language	English
Pages (from-to)	261-264
Number of pages	4
Journal	Journal of Immunological Methods
Volume	101
Issue number	2
DOIs	https://doi.org/10.1016/0022-1759(87)90158-X
State	Published - 3 Aug 1987
Externally published	Yes

Keywords

ELISA, dot-blot
Leishmania donovani
Leismaniasis, visceral
Serodiagnosis

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10.1016/0022-1759(87)90158-X

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AB - This paper describes a simple method for carrying out dot-blot immunoassays simultaneously with a large number of different sera. No dot-blot manifold is necessary, only a modified 96-well flat-bottom microtiter plate. This apparatus is re-usable, inexpensive and especially suited for field testing of patient sera in serodiagnostic assays. One application of this technique, to the serodiagnosis of visceral leishmaniasis on a pure parasite protein, is demonstrated.

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KW - Leishmania donovani

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Routine dot-blot assay of multiple serum samples using a simple apparatus

Abstract

Keywords

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