TY - JOUR
T1 - Salicylate as an in vivo free radical trap
T2 - Studies on ischemic insult to the rat intestine
AU - Udassin, Raphael
AU - Ariel, Ilana
AU - Haskel, Yuval
AU - Kitrossky, Nahum
AU - Chevion, Mordechai
PY - 1991
Y1 - 1991
N2 - Ischemia of rat intestine was induced in vivo by occlusion of the superior mesenteric artery (SMA) for 15 min. Sodium salicylate, 100 mg/kg, given IP, 30 min prior to the ischemic event served as a specific trap for hydroxyl radicals. Portions of the bowel were sequentially isolatd and removed - 2 min prior to ischemia, 2 min prior to declamping of the SMA, and 10 min following reperfusion. The bowel segments were homogenized in 3% TCA. The homogenate was centrifuged and filtrated through a 0.22 μ filter. The hydroxylation products of salicylate, dihydroxybenzoic acid (DHBA) derivatives, were isolated, identified, and quantified by HPLC coupled with electrochemical detection (ECD). The level of 2,5-DHBA (M ± SE, ng/g tissue) in the preischemic bowel (N = 21) was 241.8 ± 10.0. In the ischemic specimen the level of 2.5-DHBA increased significantly to 313.3 ± 15.5 (p = 0.0129), and remained unchanged in the reperfusion period (322.8 ± 15.5). The histological examination correlated well with these levels; mild villi damage in the ischemic period with no further exacerbation during the reperfusion period. This study in an in vivo animal model of intestinal ischemia-reperfusion provides direct evidence for the involvement of free radicals during the ischemic result.
AB - Ischemia of rat intestine was induced in vivo by occlusion of the superior mesenteric artery (SMA) for 15 min. Sodium salicylate, 100 mg/kg, given IP, 30 min prior to the ischemic event served as a specific trap for hydroxyl radicals. Portions of the bowel were sequentially isolatd and removed - 2 min prior to ischemia, 2 min prior to declamping of the SMA, and 10 min following reperfusion. The bowel segments were homogenized in 3% TCA. The homogenate was centrifuged and filtrated through a 0.22 μ filter. The hydroxylation products of salicylate, dihydroxybenzoic acid (DHBA) derivatives, were isolated, identified, and quantified by HPLC coupled with electrochemical detection (ECD). The level of 2,5-DHBA (M ± SE, ng/g tissue) in the preischemic bowel (N = 21) was 241.8 ± 10.0. In the ischemic specimen the level of 2.5-DHBA increased significantly to 313.3 ± 15.5 (p = 0.0129), and remained unchanged in the reperfusion period (322.8 ± 15.5). The histological examination correlated well with these levels; mild villi damage in the ischemic period with no further exacerbation during the reperfusion period. This study in an in vivo animal model of intestinal ischemia-reperfusion provides direct evidence for the involvement of free radicals during the ischemic result.
KW - Dihydroxybenzoates
KW - Free radicals
KW - Intestinal ischemia
KW - Salicylate
UR - http://www.scopus.com/inward/record.url?scp=0025980875&partnerID=8YFLogxK
U2 - 10.1016/0891-5849(91)90014-T
DO - 10.1016/0891-5849(91)90014-T
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C2 - 1646748
AN - SCOPUS:0025980875
SN - 0891-5849
VL - 10
SP - 1
EP - 6
JO - Free Radical Biology and Medicine
JF - Free Radical Biology and Medicine
IS - 1
ER -