Salicylate as an in vivo free radical trap: Studies on ischemic insult to the rat intestine

Raphael Udassin*, Ilana Ariel, Yuval Haskel, Nahum Kitrossky, Mordechai Chevion

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

60 Scopus citations

Abstract

Ischemia of rat intestine was induced in vivo by occlusion of the superior mesenteric artery (SMA) for 15 min. Sodium salicylate, 100 mg/kg, given IP, 30 min prior to the ischemic event served as a specific trap for hydroxyl radicals. Portions of the bowel were sequentially isolatd and removed - 2 min prior to ischemia, 2 min prior to declamping of the SMA, and 10 min following reperfusion. The bowel segments were homogenized in 3% TCA. The homogenate was centrifuged and filtrated through a 0.22 μ filter. The hydroxylation products of salicylate, dihydroxybenzoic acid (DHBA) derivatives, were isolated, identified, and quantified by HPLC coupled with electrochemical detection (ECD). The level of 2,5-DHBA (M ± SE, ng/g tissue) in the preischemic bowel (N = 21) was 241.8 ± 10.0. In the ischemic specimen the level of 2.5-DHBA increased significantly to 313.3 ± 15.5 (p = 0.0129), and remained unchanged in the reperfusion period (322.8 ± 15.5). The histological examination correlated well with these levels; mild villi damage in the ischemic period with no further exacerbation during the reperfusion period. This study in an in vivo animal model of intestinal ischemia-reperfusion provides direct evidence for the involvement of free radicals during the ischemic result.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalFree Radical Biology and Medicine
Volume10
Issue number1
DOIs
StatePublished - 1991

Keywords

  • Dihydroxybenzoates
  • Free radicals
  • Intestinal ischemia
  • Salicylate

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