Salivary glands of the sand fly Phlebotomus papatasi contain pharmacologically active amounts of adenosine and 5'-AMP

José M.C. Ribeiro*, Oren Katz, Lewis K. Pannell, John Waitumbi, Alon Warburg

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

76 Scopus citations

Abstract

Salivary gland homogenates of the sand fly Phlebotomus papatasi contain large amounts of adenosine and 5'-AMP, of the order of 1 nmol per pair of glands, as demonstrated by liquid chromatography, ultraviolet spectrometry, mass spectrometry and bioassays. These purines, 75-80% of which are secreted from the glands following a blood meal, have vasodilatory and anti-platelet activities and probably help the fly to obtain a blood meal. Salivary 5'-AMP is also responsible for the previously reported protein phosphatase inhibitor in the salivary glands of P. papatasi, which is shown to be artifactual in nature as a result of allosteric modification by AMP of the phosphatase substrate used (phosphorylase a).

Original languageAmerican English
Pages (from-to)1551-1559
Number of pages9
JournalJournal of Experimental Biology
Volume202
Issue number11
StatePublished - Jun 1999

Keywords

  • AMP
  • Adenosine
  • Aorta
  • Bioassay
  • Electrospray mass spectrometry
  • HPLC
  • Haemostasis
  • Phlebotomus papatasi
  • Phosphorylase a
  • Platelet
  • Protein phosphatase
  • Sand fly

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