Salmonella enterica Serotype 4,[5],12:i:-in Swine in the United States Midwest: An Emerging Multidrug-Resistant Clade

Ehud Elnekave*, Samuel Hong, Alison E. Mather, Dave Boxrud, Angela J. Taylor, Victoria Lappi, Timothy J. Johnson, Fabio Vannucci, Peter Davies, Craig Hedberg, Andres Perez, Julio Alvarez

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

63 Scopus citations


Background. Salmonella 4,[5],12:i:-, a worldwide emerging pathogen that causes many food-borne outbreaks mostly attributed to pig and pig products, is expanding in the United States Methods. Whole-genome sequencing was applied to conduct multiple comparisons of 659 S. 4,[5],12:i:-and 325 Salmonella Typhimurium from different sources and locations (ie, the United States and Europe) to assess their genetic heterogeneity, with a focus on strains recovered from swine in the US Midwest. In addition, the presence of resistance genes and other virulence factors was detected and the antimicrobial resistance phenotypes of 50 and 22 isolates of livestock and human origin, respectively, was determined. Results. Te S. 4,5,12:i:-strains formed two main clades regardless of their source and geographic origin. Most (84%) of the US isolates recovered in 2014-2016, including those (48 of 51) recovered from swine in the US Midwest, were part of an emerging clade. In this clade, multiple genotypic resistance determinants were predominant, including resistance against ampicillin, streptomycin, sulfonamides, and tetracyclines. Phenotypic resistance to enrofloxacin (11 of 50) and cefiofur (9 of 50) was found in conjunction with the presence of plasmid-mediated resistance genes (qnrB19/qnrB2/qnrS1 and blaCMY-2/blaSHV-12, respectively). Higher similarity was also found between S. 4,[5],12:i:-from the emerging clade and S. Typhimurium from Europe than with S. Typhimurium from the United States. Conclusions. Salmonella 4,[5],12:i:-currently circulating in swine in the US Midwest are likely to be part of an emerging multidrug-resistant clade frst reported in Europe, and can carry plasmid-mediated resistance genes that may be transmitted horizontally to other bacteria, and thus may represent a public health concern.

Original languageAmerican English
Pages (from-to)877-885
Number of pages9
JournalClinical Infectious Diseases
Issue number6
StatePublished - 5 Mar 2018
Externally publishedYes

Bibliographical note

Funding Information:
Soyer et al [15] grouped S. 4,[5],12:i:-from the United States and Spain into separate clades and suggested that these clades emerged from different ancestral S. Typhimurium. The existence of distinct clades was also supported by different antimicrobial resistance (AMR) profiles of isolates originating from the United States and Europe. Multidrug resistance (ie, resistance to ≥3 antimicrobial classes) was rare in United States strains from humans [3, 12], but resistance to ampicillin, streptomycin, sulfonamides, and tetracycline (referred to as ASSuT) was prevalent in European strains from human, pigs, and pig products [5, 8, 10, 14]. Two mutually exclusive resistance mechanisms located in different regions of the genome, RR1&RR2 [16] and RR3 [17], conferred the ASSuT phenotype in European isolates. In addition, resistance to chloramphenicol, gentamicin, and trimethoprim-sulfamethoxazole was found in isolates collected in Spain [5, 8] and in the United Kingdom and Italy [9]. In the isolates from the United Kingdom and Italy, several virulence factors (eg, sopE and SGI-3) were also described.

Funding Information:
This work was supported by the Global Food Venture-MnDrive initiative, the USDA National Institute of Food and Agriculture, (Animal Health Formula Fund project MIN-62-091) and the Swine Disease Eradication Center at the University of Minnesota. In addition, the Biotechnology and Biological Sciences Research Council (fellowship BB/M014088/1 to A. E. M.).

Publisher Copyright:
© 2017 The Author(s).


  • AMR
  • MDR
  • Monophasic
  • Pig
  • Quinolones


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