TY - JOUR
T1 - Salmonella typhimurium invasion of epithelial cells
T2 - Role of induced host cell tyrosine protein phosphorylation
AU - Rosenshine, I.
AU - Ruschkowski, S.
AU - Foubister, V.
AU - Finlay, B. B.
PY - 1994
Y1 - 1994
N2 - Salmonella typhimurium invades nonphagocytic epithelial and fibroblast cells via a process resembling phagocytosis. We have compared some phenotypes that are involved in S. typhimurium invasion by using different host cell lines, including HeLa, Henle-407, and A431. Infection with either wild-type S. typhimurium, bacterial culture supernatant, or the noninvasive invA mutant was associated with induction of tyrosine phosphorylation of host cell mitogenic activating protein kinase. However, we did not detect induction of tyrosine phosphorylation of the epidermal growth factor receptor in S. typhimurium-infected cells. Treatment with the tyrosine protein kinase inhibitor genistein did not reduce S. typhimurium invasion into any of these cell lines. These results suggest that S. typhimurium invasion is independent of host cell epidermal growth factor receptor activation.
AB - Salmonella typhimurium invades nonphagocytic epithelial and fibroblast cells via a process resembling phagocytosis. We have compared some phenotypes that are involved in S. typhimurium invasion by using different host cell lines, including HeLa, Henle-407, and A431. Infection with either wild-type S. typhimurium, bacterial culture supernatant, or the noninvasive invA mutant was associated with induction of tyrosine phosphorylation of host cell mitogenic activating protein kinase. However, we did not detect induction of tyrosine phosphorylation of the epidermal growth factor receptor in S. typhimurium-infected cells. Treatment with the tyrosine protein kinase inhibitor genistein did not reduce S. typhimurium invasion into any of these cell lines. These results suggest that S. typhimurium invasion is independent of host cell epidermal growth factor receptor activation.
UR - http://www.scopus.com/inward/record.url?scp=0028104144&partnerID=8YFLogxK
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C2 - 7523303
AN - SCOPUS:0028104144
SN - 0019-9567
VL - 62
SP - 4969
EP - 4974
JO - Infection and Immunity
JF - Infection and Immunity
IS - 11
ER -