Scaled-up proliferation and regeneration of Nerine in liquid cultures Part II. Ontogeny of somatic embryos and bulblet regeneration

Hannah Lilien-Kipnis*, Na'za Azizbekova, Meira Ziv

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

The ontogeny of somatic embryos was followed in liquid cultured Nerine tissue. Proliferating, nodular meristematic clusters were maintained in bubble bioreactors in a medium supplemented with 0.25 μM 1-naphthalene acetic acid (NAA), 10 μM 6-benzyladenine (BA) and 8.7 μM Paclobutrazol (PAC). Regeneration of plantlets from this tissue was limited. Omission of PAC from the medium induced proembryogenic clusters. Embryo development and maturation were enhanced in flask cultures by substituting N6-(isopentenyl) adenine (2iP) for BA and elevating the sucrose concentration in the medium to 6%. High rates of embryo germination occurred in a growth regulator-free, low (3%) sucrose medium. Bulblet-bearing plantlets developed on agar-solidified, auxin-supplemented media. Recurrent embryogenesis occurred in long term growth regulator-free, or high sucrose media. The potential of using the somatic embryogenesis pathway for micropropagation of Nerines is described.

Original languageEnglish
Pages (from-to)117-123
Number of pages7
JournalPlant Cell, Tissue and Organ Culture
Volume39
Issue number2
DOIs
StatePublished - Nov 1994

Keywords

  • Amaryllidaceae
  • micro-propagation
  • Paclobutrazol
  • secondary embryogenesis

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