TY - JOUR
T1 - Scaled-up proliferation and regeneration of Nerine in liquid cultures Part II. Ontogeny of somatic embryos and bulblet regeneration
AU - Lilien-Kipnis, Hannah
AU - Azizbekova, Na'za
AU - Ziv, Meira
PY - 1994/11
Y1 - 1994/11
N2 - The ontogeny of somatic embryos was followed in liquid cultured Nerine tissue. Proliferating, nodular meristematic clusters were maintained in bubble bioreactors in a medium supplemented with 0.25 μM 1-naphthalene acetic acid (NAA), 10 μM 6-benzyladenine (BA) and 8.7 μM Paclobutrazol (PAC). Regeneration of plantlets from this tissue was limited. Omission of PAC from the medium induced proembryogenic clusters. Embryo development and maturation were enhanced in flask cultures by substituting N6-(isopentenyl) adenine (2iP) for BA and elevating the sucrose concentration in the medium to 6%. High rates of embryo germination occurred in a growth regulator-free, low (3%) sucrose medium. Bulblet-bearing plantlets developed on agar-solidified, auxin-supplemented media. Recurrent embryogenesis occurred in long term growth regulator-free, or high sucrose media. The potential of using the somatic embryogenesis pathway for micropropagation of Nerines is described.
AB - The ontogeny of somatic embryos was followed in liquid cultured Nerine tissue. Proliferating, nodular meristematic clusters were maintained in bubble bioreactors in a medium supplemented with 0.25 μM 1-naphthalene acetic acid (NAA), 10 μM 6-benzyladenine (BA) and 8.7 μM Paclobutrazol (PAC). Regeneration of plantlets from this tissue was limited. Omission of PAC from the medium induced proembryogenic clusters. Embryo development and maturation were enhanced in flask cultures by substituting N6-(isopentenyl) adenine (2iP) for BA and elevating the sucrose concentration in the medium to 6%. High rates of embryo germination occurred in a growth regulator-free, low (3%) sucrose medium. Bulblet-bearing plantlets developed on agar-solidified, auxin-supplemented media. Recurrent embryogenesis occurred in long term growth regulator-free, or high sucrose media. The potential of using the somatic embryogenesis pathway for micropropagation of Nerines is described.
KW - Amaryllidaceae
KW - micro-propagation
KW - Paclobutrazol
KW - secondary embryogenesis
UR - http://www.scopus.com/inward/record.url?scp=0001049164&partnerID=8YFLogxK
U2 - 10.1007/BF00033919
DO - 10.1007/BF00033919
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AN - SCOPUS:0001049164
SN - 0167-6857
VL - 39
SP - 117
EP - 123
JO - Plant Cell, Tissue and Organ Culture
JF - Plant Cell, Tissue and Organ Culture
IS - 2
ER -