Scanning electron microscopy of freeze-fractured sclerotia of Physarum polycephalum

N. Kislev*, I. Chet

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The ultrastructure and architecture of freeze-fractured sclerotia of Physarum polycephalum was studied by a scanning electron microscope (SEM). The sclerotia are built of many spherules grouped together in a common outer coat. Each spherule has hard walls which separate it from its neighbors. The spherules are rounded in 2-day-old sclerotia, and have a lobe-like structure 3 weeks later. A new simple technique for obtaining freeze-fractured biological material is described and compared with freeze-fracture in a freeze-etching apparatus. The ultrastructural details of the fractured sclerotia are described.

Original languageEnglish
Pages (from-to)209-214
Number of pages6
JournalTissue and Cell
Volume6
Issue number2
DOIs
StatePublished - 1974

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