SCARECROW-like GRAS protein PES positively regulates petunia floral scent production

Ekaterina Shor, Jasmin Ravid, Elad Sharon, Oded Skaliter, Tania Masci, Alexander Vainstein*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Emission of scent volatiles by flowers is important for successful pollination and consequently, reproduction. Petunia (Petunia hybrida) floral scent is formed mainly by volatile products of the phenylpropanoid pathway. We identified and characterized a regulator of petunia scent production: the GRAS protein PHENYLPROPANOID EMISSION-REGULATING SCARECROW-LIKE (PES). Its expression increased in petals during bud development and was highest in open flowers. Overexpression of PES increased the production of floral volatiles, while its suppression resulted in scent reduction. We showed that PES upregulates the expression of genes encoding enzymes of the phenylpropanoid and shikimate pathways in petals, and of the core regulator of volatile biosynthesis ODORANT1 by activating its promoter. PES is an ortholog of Arabidopsis (Arabidopsis thaliana) PHYTOCHROME A SIGNAL TRANSDUCTION 1, involved in physiological responses to far-red (FR) light. Analyses of the effect of nonphotosynthetic irradiation (low-intensity FR light) on petunia floral volatiles revealed FR light as a scent-activating factor. While PHYTOCHROME A regulated scent-related gene expression and floral scent production under FR light, the influence of PES on volatile production was not limited by FR light conditions.

Original languageAmerican English
Pages (from-to)409-425
Number of pages17
JournalPlant Physiology
Volume192
Issue number1
DOIs
StatePublished - May 2023

Bibliographical note

Publisher Copyright:
© American Society of Plant Biologists 2023. All rights reserved.

Fingerprint

Dive into the research topics of 'SCARECROW-like GRAS protein PES positively regulates petunia floral scent production'. Together they form a unique fingerprint.

Cite this