Schistosoma haematobium detection in snails by DraI PCR and Sh110/Sm-Sl PCR: Further evidence of the interruption of schistosomiasis transmission in Morocco: Further evidence of the interruption of schistosomiasis transmission in Morocco

F. Amarir; F. Sebti; I. Abbasi; A. Sadak; H. Fellah; H. Nhammi; B. Ameur; A.L. El Idrissi; M. Rhajaoui

doi:10.1186/1756-3305-7-288

Schistosoma haematobium detection in snails by DraI PCR and Sh110/Sm-Sl PCR: Further evidence of the interruption of schistosomiasis transmission in Morocco: Further evidence of the interruption of schistosomiasis transmission in Morocco

F. Amarir, F. Sebti, I. Abbasi, A. Sadak, H. Fellah, H. Nhammi, B. Ameur, A.L. El Idrissi, M. Rhajaoui

Department of Microbiology and Molecular Genetics

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Background: This is the first study in Morocco to estimate snail infection rates at the last historic transmission sites of schistosomiasis, known to be free from new infection among humans since 2004. Screening of large numbers of snails for infection is one way to confirm that Schistosoma haematobium transmission has stopped and does not resurge. Methods. A total of 2703 Bulinus truncatus snails were collected from 24 snail habitats in five provinces of Morocco: Errachidia, El Kelaa des Sraghna, Tata, Beni Mellal, and Chtouka Ait Baha. All visible snails were collected with a scoop net or by hand. We used waders and gloves as simple precautions. Snails were morphologically identified according to Moroccan Health Ministry guide of schistosomiasis (1982).All snails were analyzed in pools by molecular tool, using primers from the newly identified repeated DNA sequence, termed DraI, in the S. haematobium group. To distinguish S. bovis and S. haematobium, the snails were analyzed by Sh110/Sm-Sl PCR that was specific of S. haematobium. Results: The results showed that snails from Errachidia, Chtouka Ait Baha, sector of Agoujgal in Tata and sector of Mbarkiya in El kelaa des Sraghna were negative for DraI PCR; but, snails from remaining snail habitats of El Kelaa des Sraghna, Tata and Beni Mellal were positive. This led to suggest the presence of circulating schistosome species (S. haematobium, S. bovis or others) within these positive snail habitats. Subsequently, confirmation with S. haematobium species specific molecular assay, Sh110/Sm-Sl PCR, showed that none of the collected snails were infected by S. haematobium in all historic endemic areas. Conclusion: The absence of S. haematobium infection in snails supports the argument of S. haematobium transmission interruption in Morocco. © 2014 Amarir et al.; licensee BioMed Central Ltd.

Original language	English
Article number	288
Journal	Parasites and Vectors
Volume	7
Issue number	1
DOIs	https://doi.org/10.1186/1756-3305-7-288
State	Published - 2014

Bibliographical note

Cited By :26

Export Date: 30 January 2023

Correspondence Address: Amarir, F.; Laboratory of Parasitology, Department of Parasitology, National Institute of Hygiene, Agdal, Rabat, Morocco; email: Fatima_inh@yahoo.fr

Funding details: World Health Organization, WHO, 2 008/237-0

Funding text 1: This study was supported by the World Health Organization (Reference no. 2 008/237-0).

References: Barkia, H., Barkia, A., Nhammi, H., Belghyti, D., La schistosomiase au Maroc: de sa découverte à l'après-élimination (2011) East Mediterr Health J, 17, p. 3; Rollinson, D., Knopp, S., Levitz, S., Stothard, J.R., Tchuem Tchuenté, L.A., Garba, A., Mohammed, K.A., Utzinger, J., Time to set the agenda for schistosomiasis elimination (2013) Acta Trop, 128 (2), pp. 423-440. , 10.1016/j.actatropica.2012.04.013 22580511; Rapport d'activité annuel des programmes de lutte contre les maladies parasitaires (1983) Bull Épidémiologique, 1, pp. 11-14. , Ministry of Health Direction of Epidemiology control of Diseases Morocco; Rapport d'activité annuel des programmes de lutte contre les maladies parasitaires (2013) Bull Épidémiologique, 1, p. 28. , Ministry of Health Direction of Epidemiology control of Diseases Morocco; Etat d'avancement des programmes de lutte contre les maladies parasitaires (2006) Bull Épidémiologique, 1, p. 45. , Ministry of Health Direction of Epidemiology control of Diseases Morocco; Amarir, F., El Mansouri, B., Fellah, H., Sebti, F., Lakranbi, M., Laamrani El Idrissi, A., Sadak, A., Rhajaoui, M., National Serological Survey of Haematobium Schistosomiasis in Morocco: Arguments to explain the elimination (2011) Am J Trop Med Hyg, 1, pp. 15-19; Abbasi, I., King, C.H., Muchiri, E.M., Hamburger, J., Detection of Schistosoma mansoni and Schistosoma haematobium DNA by loop-mediated isothermal amplification: Identification of infected snails from early prepatency (2010) Am J Trop Med Hyg, 83, pp. 427-432. , 10.4269/ajtmh.2010.09-0764 20682894; Hamburger, J., Hoffman, O., Kariuki, H.C., Muchiri, E.M., Ouma, J.H., Koech, D.K., Sturrock, R.F., King, C.H., Large-scale, polymerase chain reaction-based surveillance of Schistosoma haematobium DNA in snails from transmission sites in coastal Kenya: A new tool for studying the dynamics of snail infection (2004) American Journal of Tropical Medicine and Hygiene, 71 (6), pp. 765-773; Hambourger, J., Schistosoma mansoni antigene recognized in Biomphalaria glabrata hemolymphe by monoclonal antibodies (1989) Am J Tropical Med Hygiene, 40, pp. 605-612; Abassi, I., Charles, K., Robert, F.S., Curtis, K., Eric, M., Joseph, H., Differentiation of Schistosoma haematobium from related schistosomes by PCR amplifying an inter repeat sequence (2007) Am J Trop Med Hyg, 76, pp. 950-955. , 17488921; Melo, F.L., Gomes, A.L.D.V., Barbosa, C.S., Werkhauser, R.P., Abath, F.G.C., Development of molecular approaches for the identification of transmission sites of schistosomiasis (2006) Transactions of the Royal Society of Tropical Medicine and Hygiene, 100 (11), pp. 1049-1055. , DOI 10.1016/j.trstmh.2005.12.008, PII S0035920306000381; Lambrechts, L., Fellous, S., Koella, J.C., Coevolutionary interactions between host and parasite genotypes (2006) Trends in Parasitology, 22 (1), pp. 12-16. , DOI 10.1016/j.pt.2005.11.008, PII S1471492205003144; Rapport d'activité annuel des programmes de lutte contre les maladies parasitaires (2003) Bull Épidémiologique, 1, p. 25. , Ministry of Health Direction of Epidemiology control of Diseases Morocco; Rapport d'activité annuel des programmes de lutte contre les maladies parasitaires (2004) Bull Épidémiologique, 1, p. 31. , Ministry of Health Direction of Epidemiology control of Diseases Morocco; Rapport d'activité annuel des programmes de lutte contre les maladies parasitaires (2001) Bull Épidémiologique, 1, p. 31. , Ministry of Health Direction of Epidemiology control of Diseases Morocco; Rapport d'activité annuel des programmes de lutte contre les maladies parasitaires (2000) Bull Épidémiologique, 1, pp. 41-42. , Ministry of Health Direction of Epidemiology control of Diseases Morocco; Moussa, A.H., Abou El-Hassan, A.A., The effect of water temperature on the snail intermediate hosts of schistosomiasis in Egypt (1972) Egyptian Med Soc, 55, pp. 148-165; Hamburger, J., He-Na, Abbasi, I., Ramzy, R.M., Jourdane, J., Ruppel, A., Polymerase chain reaction assay based on a highly repeated sequence of Schistosoma haematobium: A potential tool for monitoring schistosome-infested water (2001) American Journal of Tropical Medicine and Hygiene, 65 (6), pp. 907-911; Hamburger, J., He-Na, Xu, Y.X., Ramzy, R.M., Jourdane, J., Ruppel, A., A polymerase chain reaction assay for detecting snails infected with Bilharzia parasites (Schistosoma mansoni) from very early prepatency (1998) American Journal of Tropical Medicine and Hygiene, 59 (6), pp. 872-876; Christensen, N.O., Mutani, A., Frandsen, F., A review of the biology and transmission ecology of African bovine species of the genus Schistosoma (1983) Zeitschrift fur Parasitenkunde, 69 (5), pp. 551-570. , DOI 10.1007/BF00926667; Mone, H., Mowahid, G., Monard, S., The distribution of Schistosoma bovis Sonsino, 1876 in relation to intermediate host mollusc-parasite relationship (2000) Adv Parasitol, 44, pp. 100-138; Vercruysse, J., Southgate, V.R., Rollinson, D., De Clercq, D., Sacko, M., De Bont, J., Mungomba, L.M., Studies on transmission and schistosome interactions in Senegal, Mali and Zambia (1994) Tropical and Geographical Medicine, 46 (4), pp. 220-226; Jourdane, J., Southgate, V.R., Pages, J.R., Durand, P., Tchuem Tchuente, L.A., Recent Studies on Schistosoma intercalatum: Taxonomic Status, Puzzling Distribution and Transmission Foci Revisited (2001) Memorias do Instituto Oswaldo Cruz, 96 (SUPPL.), pp. 45-48; Webster, B.L., Rollinson, D., Stothard, J.R., Huyse, T., Rapid diagnostic multiplex PCR (RD-PCR) to discriminate Schistosoma haematobium and S. Bovis (2010) J Helminthol, 84, pp. 107-114. , 10.1017/S0022149X09990447 19646307; Abbasi, I., Hamburger, J., Kariuki, C., Mungai, P.L., Muchiri, E.M., King, C.H., Differentiating Schistosoma haematobium from Related Animal Schistosomes by PCR. Amplifying Inter-Repeat Sequences Flanking Newly Selected Repeated Sequences (2012) Am J Trop Med Hyg, 87 (6), pp. 1059-1064. , 10.4269/ajtmh.2012.12-0243 23109375; Huyse, T., Webster, B.L., Geldof, S., Stothard, R.J., Diaw, O.T., Polman, K., Rollinson, D., Bidirectional introgressive hybridization between a cattle and human schistosome species (2009) PLoS Pathog, 5 (9), p. 51000571. , 10.1371/journal.ppat.1000571 19730700; Elimination of schistosomiasis from low-transmission areas (2008) Report of Who Informal Consultation, pp. 1-21. , WHO Salvador, Brazil; Sturrock, R.F., Snail collection to detect schistosome transmission sites (1986) Parasitol Today, 2, pp. 59-63. , 10.1016/0169-4758(86)90155-9 15462771; Yacoubi, B., Zekhnini, A., Moukrim, A., Rondelaud, D., Bulins, planorbes et endémie bilharzienne dans le sud-ouest marocain (2007) Bull Soc Pathol Exot, 100, pp. 174-175. , 17824308; Zhou, Y.B., Liang, S., Jiang, Q.W., Factors impacting on progress towards elimination of transmission of schistosomiasis japonica in China (2012) Parasites Vectors, 5, p. 275. , 10.1186/1756-3305-5-275 23206326; Hua, H.Y., Wang, W., Cao, G.Q., Tang, F., Liang, Y.S., Improving the management of imported schistosomiasis haematobia in China: Lessons from a case with multiple misdiagnoses (2013) Parasites Vectors, 6, p. 260. , 10.1186/1756-3305-6-260 24020375; Glenn, C.T., Lance, S.L., McKee, A.M., Webster, B.L., Emery, A.M., Zerlotini, A., Oliveira, G., Faircloth, B.C., Significant variance in genetic diversity among populations of Schistosoma haematobium detected using microsatellite DNA loci from a genome-wide database (2013) Parasites Vectors, 6, p. 300. , 10.1186/1756-3305-6-300 24499537; Utzinger, J., N'Goran, E.K., Caffreye, C.R., Rkeiser, J., From innovation to application: Social-ecological context, diagnostics, drugs and integrated control of schistosomiasis (2011) Acta Trop, 120, pp. 121-137

Keywords

Bulinus truncatus
DraI PCR
Elimination
Morocco
Schistosoma haematobium
Schistosomiasis
Sh110/Sm-Sl PCR
repetitive DNA
article
controlled study
DNA extraction
habitat
microorganism detection
nonhuman
parasite transmission
polymerase chain reaction
Schistosoma hematobium
schistosomiasis
sensitivity and specificity
snail
animal
isolation and purification
parasitology
procedures
schistosomiasis haematobia
transmission
Animals
Polymerase Chain Reaction
Schistosomiasis haematobia
Sensitivity and Specificity
Snails

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Amarir, F., Sebti, F., Abbasi, I., Sadak, A., Fellah, H., Nhammi, H., Ameur, B., El Idrissi, A. L., & Rhajaoui, M. (2014). Schistosoma haematobium detection in snails by DraI PCR and Sh110/Sm-Sl PCR: Further evidence of the interruption of schistosomiasis transmission in Morocco: Further evidence of the interruption of schistosomiasis transmission in Morocco. Parasites and Vectors, 7(1), Article 288. https://doi.org/10.1186/1756-3305-7-288

@article{46016e8f95a74433b778a8456d0b0587,

title = "Schistosoma haematobium detection in snails by DraI PCR and Sh110/Sm-Sl PCR: Further evidence of the interruption of schistosomiasis transmission in Morocco: Further evidence of the interruption of schistosomiasis transmission in Morocco",

abstract = "Background: This is the first study in Morocco to estimate snail infection rates at the last historic transmission sites of schistosomiasis, known to be free from new infection among humans since 2004. Screening of large numbers of snails for infection is one way to confirm that Schistosoma haematobium transmission has stopped and does not resurge. Methods. A total of 2703 Bulinus truncatus snails were collected from 24 snail habitats in five provinces of Morocco: Errachidia, El Kelaa des Sraghna, Tata, Beni Mellal, and Chtouka Ait Baha. All visible snails were collected with a scoop net or by hand. We used waders and gloves as simple precautions. Snails were morphologically identified according to Moroccan Health Ministry guide of schistosomiasis (1982).All snails were analyzed in pools by molecular tool, using primers from the newly identified repeated DNA sequence, termed DraI, in the S. haematobium group. To distinguish S. bovis and S. haematobium, the snails were analyzed by Sh110/Sm-Sl PCR that was specific of S. haematobium. Results: The results showed that snails from Errachidia, Chtouka Ait Baha, sector of Agoujgal in Tata and sector of Mbarkiya in El kelaa des Sraghna were negative for DraI PCR; but, snails from remaining snail habitats of El Kelaa des Sraghna, Tata and Beni Mellal were positive. This led to suggest the presence of circulating schistosome species (S. haematobium, S. bovis or others) within these positive snail habitats. Subsequently, confirmation with S. haematobium species specific molecular assay, Sh110/Sm-Sl PCR, showed that none of the collected snails were infected by S. haematobium in all historic endemic areas. Conclusion: The absence of S. haematobium infection in snails supports the argument of S. haematobium transmission interruption in Morocco. {\textcopyright} 2014 Amarir et al.; licensee BioMed Central Ltd.",

keywords = "Bulinus truncatus, DraI PCR, Elimination, Morocco, Schistosoma haematobium, Schistosomiasis, Sh110/Sm-Sl PCR, repetitive DNA, article, controlled study, DNA extraction, habitat, microorganism detection, nonhuman, parasite transmission, polymerase chain reaction, Schistosoma hematobium, schistosomiasis, sensitivity and specificity, snail, animal, isolation and purification, parasitology, procedures, schistosomiasis haematobia, transmission, Animals, Polymerase Chain Reaction, Schistosomiasis haematobia, Sensitivity and Specificity, Snails",

author = "F. Amarir and F. Sebti and I. Abbasi and A. Sadak and H. Fellah and H. Nhammi and B. Ameur and {El Idrissi}, A.L. and M. Rhajaoui",

note = "Cited By :26 Export Date: 30 January 2023 Correspondence Address: Amarir, F.; Laboratory of Parasitology, Department of Parasitology, National Institute of Hygiene, Agdal, Rabat, Morocco; email: Fatima_inh@yahoo.fr Funding details: World Health Organization, WHO, 2 008/237-0 Funding text 1: This study was supported by the World Health Organization (Reference no. 2 008/237-0). References: Barkia, H., Barkia, A., Nhammi, H., Belghyti, D., La schistosomiase au Maroc: de sa d{\'e}couverte {\`a} l'apr{\`e}s-{\'e}limination (2011) East Mediterr Health J, 17, p. 3; Rollinson, D., Knopp, S., Levitz, S., Stothard, J.R., Tchuem Tchuent{\'e}, L.A., Garba, A., Mohammed, K.A., Utzinger, J., Time to set the agenda for schistosomiasis elimination (2013) Acta Trop, 128 (2), pp. 423-440. , 10.1016/j.actatropica.2012.04.013 22580511; Rapport d'activit{\'e} annuel des programmes de lutte contre les maladies parasitaires (1983) Bull {\'E}pid{\'e}miologique, 1, pp. 11-14. , Ministry of Health Direction of Epidemiology control of Diseases Morocco; Rapport d'activit{\'e} annuel des programmes de lutte contre les maladies parasitaires (2013) Bull {\'E}pid{\'e}miologique, 1, p. 28. , Ministry of Health Direction of Epidemiology control of Diseases Morocco; Etat d'avancement des programmes de lutte contre les maladies parasitaires (2006) Bull {\'E}pid{\'e}miologique, 1, p. 45. , Ministry of Health Direction of Epidemiology control of Diseases Morocco; Amarir, F., El Mansouri, B., Fellah, H., Sebti, F., Lakranbi, M., Laamrani El Idrissi, A., Sadak, A., Rhajaoui, M., National Serological Survey of Haematobium Schistosomiasis in Morocco: Arguments to explain the elimination (2011) Am J Trop Med Hyg, 1, pp. 15-19; Abbasi, I., King, C.H., Muchiri, E.M., Hamburger, J., Detection of Schistosoma mansoni and Schistosoma haematobium DNA by loop-mediated isothermal amplification: Identification of infected snails from early prepatency (2010) Am J Trop Med Hyg, 83, pp. 427-432. , 10.4269/ajtmh.2010.09-0764 20682894; Hamburger, J., Hoffman, O., Kariuki, H.C., Muchiri, E.M., Ouma, J.H., Koech, D.K., Sturrock, R.F., King, C.H., Large-scale, polymerase chain reaction-based surveillance of Schistosoma haematobium DNA in snails from transmission sites in coastal Kenya: A new tool for studying the dynamics of snail infection (2004) American Journal of Tropical Medicine and Hygiene, 71 (6), pp. 765-773; Hambourger, J., Schistosoma mansoni antigene recognized in Biomphalaria glabrata hemolymphe by monoclonal antibodies (1989) Am J Tropical Med Hygiene, 40, pp. 605-612; Abassi, I., Charles, K., Robert, F.S., Curtis, K., Eric, M., Joseph, H., Differentiation of Schistosoma haematobium from related schistosomes by PCR amplifying an inter repeat sequence (2007) Am J Trop Med Hyg, 76, pp. 950-955. , 17488921; Melo, F.L., Gomes, A.L.D.V., Barbosa, C.S., Werkhauser, R.P., Abath, F.G.C., Development of molecular approaches for the identification of transmission sites of schistosomiasis (2006) Transactions of the Royal Society of Tropical Medicine and Hygiene, 100 (11), pp. 1049-1055. , DOI 10.1016/j.trstmh.2005.12.008, PII S0035920306000381; Lambrechts, L., Fellous, S., Koella, J.C., Coevolutionary interactions between host and parasite genotypes (2006) Trends in Parasitology, 22 (1), pp. 12-16. , DOI 10.1016/j.pt.2005.11.008, PII S1471492205003144; Rapport d'activit{\'e} annuel des programmes de lutte contre les maladies parasitaires (2003) Bull {\'E}pid{\'e}miologique, 1, p. 25. , Ministry of Health Direction of Epidemiology control of Diseases Morocco; Rapport d'activit{\'e} annuel des programmes de lutte contre les maladies parasitaires (2004) Bull {\'E}pid{\'e}miologique, 1, p. 31. , Ministry of Health Direction of Epidemiology control of Diseases Morocco; Rapport d'activit{\'e} annuel des programmes de lutte contre les maladies parasitaires (2001) Bull {\'E}pid{\'e}miologique, 1, p. 31. , Ministry of Health Direction of Epidemiology control of Diseases Morocco; Rapport d'activit{\'e} annuel des programmes de lutte contre les maladies parasitaires (2000) Bull {\'E}pid{\'e}miologique, 1, pp. 41-42. , Ministry of Health Direction of Epidemiology control of Diseases Morocco; Moussa, A.H., Abou El-Hassan, A.A., The effect of water temperature on the snail intermediate hosts of schistosomiasis in Egypt (1972) Egyptian Med Soc, 55, pp. 148-165; Hamburger, J., He-Na, Abbasi, I., Ramzy, R.M., Jourdane, J., Ruppel, A., Polymerase chain reaction assay based on a highly repeated sequence of Schistosoma haematobium: A potential tool for monitoring schistosome-infested water (2001) American Journal of Tropical Medicine and Hygiene, 65 (6), pp. 907-911; Hamburger, J., He-Na, Xu, Y.X., Ramzy, R.M., Jourdane, J., Ruppel, A., A polymerase chain reaction assay for detecting snails infected with Bilharzia parasites (Schistosoma mansoni) from very early prepatency (1998) American Journal of Tropical Medicine and Hygiene, 59 (6), pp. 872-876; Christensen, N.O., Mutani, A., Frandsen, F., A review of the biology and transmission ecology of African bovine species of the genus Schistosoma (1983) Zeitschrift fur Parasitenkunde, 69 (5), pp. 551-570. , DOI 10.1007/BF00926667; Mone, H., Mowahid, G., Monard, S., The distribution of Schistosoma bovis Sonsino, 1876 in relation to intermediate host mollusc-parasite relationship (2000) Adv Parasitol, 44, pp. 100-138; Vercruysse, J., Southgate, V.R., Rollinson, D., De Clercq, D., Sacko, M., De Bont, J., Mungomba, L.M., Studies on transmission and schistosome interactions in Senegal, Mali and Zambia (1994) Tropical and Geographical Medicine, 46 (4), pp. 220-226; Jourdane, J., Southgate, V.R., Pages, J.R., Durand, P., Tchuem Tchuente, L.A., Recent Studies on Schistosoma intercalatum: Taxonomic Status, Puzzling Distribution and Transmission Foci Revisited (2001) Memorias do Instituto Oswaldo Cruz, 96 (SUPPL.), pp. 45-48; Webster, B.L., Rollinson, D., Stothard, J.R., Huyse, T., Rapid diagnostic multiplex PCR (RD-PCR) to discriminate Schistosoma haematobium and S. Bovis (2010) J Helminthol, 84, pp. 107-114. , 10.1017/S0022149X09990447 19646307; Abbasi, I., Hamburger, J., Kariuki, C., Mungai, P.L., Muchiri, E.M., King, C.H., Differentiating Schistosoma haematobium from Related Animal Schistosomes by PCR. Amplifying Inter-Repeat Sequences Flanking Newly Selected Repeated Sequences (2012) Am J Trop Med Hyg, 87 (6), pp. 1059-1064. , 10.4269/ajtmh.2012.12-0243 23109375; Huyse, T., Webster, B.L., Geldof, S., Stothard, R.J., Diaw, O.T., Polman, K., Rollinson, D., Bidirectional introgressive hybridization between a cattle and human schistosome species (2009) PLoS Pathog, 5 (9), p. 51000571. , 10.1371/journal.ppat.1000571 19730700; Elimination of schistosomiasis from low-transmission areas (2008) Report of Who Informal Consultation, pp. 1-21. , WHO Salvador, Brazil; Sturrock, R.F., Snail collection to detect schistosome transmission sites (1986) Parasitol Today, 2, pp. 59-63. , 10.1016/0169-4758(86)90155-9 15462771; Yacoubi, B., Zekhnini, A., Moukrim, A., Rondelaud, D., Bulins, planorbes et end{\'e}mie bilharzienne dans le sud-ouest marocain (2007) Bull Soc Pathol Exot, 100, pp. 174-175. , 17824308; Zhou, Y.B., Liang, S., Jiang, Q.W., Factors impacting on progress towards elimination of transmission of schistosomiasis japonica in China (2012) Parasites Vectors, 5, p. 275. , 10.1186/1756-3305-5-275 23206326; Hua, H.Y., Wang, W., Cao, G.Q., Tang, F., Liang, Y.S., Improving the management of imported schistosomiasis haematobia in China: Lessons from a case with multiple misdiagnoses (2013) Parasites Vectors, 6, p. 260. , 10.1186/1756-3305-6-260 24020375; Glenn, C.T., Lance, S.L., McKee, A.M., Webster, B.L., Emery, A.M., Zerlotini, A., Oliveira, G., Faircloth, B.C., Significant variance in genetic diversity among populations of Schistosoma haematobium detected using microsatellite DNA loci from a genome-wide database (2013) Parasites Vectors, 6, p. 300. , 10.1186/1756-3305-6-300 24499537; Utzinger, J., N'Goran, E.K., Caffreye, C.R., Rkeiser, J., From innovation to application: Social-ecological context, diagnostics, drugs and integrated control of schistosomiasis (2011) Acta Trop, 120, pp. 121-137",

year = "2014",

doi = "10.1186/1756-3305-7-288",

language = "אנגלית",

volume = "7",

journal = "Parasites and Vectors",

issn = "1756-3305",

publisher = "BioMed Central Ltd.",

number = "1",

}

Amarir, F, Sebti, F, Abbasi, I, Sadak, A, Fellah, H, Nhammi, H, Ameur, B, El Idrissi, AL & Rhajaoui, M 2014, 'Schistosoma haematobium detection in snails by DraI PCR and Sh110/Sm-Sl PCR: Further evidence of the interruption of schistosomiasis transmission in Morocco: Further evidence of the interruption of schistosomiasis transmission in Morocco', Parasites and Vectors, vol. 7, no. 1, 288. https://doi.org/10.1186/1756-3305-7-288

Schistosoma haematobium detection in snails by DraI PCR and Sh110/Sm-Sl PCR: Further evidence of the interruption of schistosomiasis transmission in Morocco: Further evidence of the interruption of schistosomiasis transmission in Morocco. / Amarir, F.; Sebti, F.; Abbasi, I. et al.
In: Parasites and Vectors, Vol. 7, No. 1, 288, 2014.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Schistosoma haematobium detection in snails by DraI PCR and Sh110/Sm-Sl PCR: Further evidence of the interruption of schistosomiasis transmission in Morocco

T2 - Further evidence of the interruption of schistosomiasis transmission in Morocco

AU - Amarir, F.

AU - Sebti, F.

AU - Abbasi, I.

AU - Sadak, A.

AU - Fellah, H.

AU - Nhammi, H.

AU - Ameur, B.

AU - El Idrissi, A.L.

AU - Rhajaoui, M.

N1 - Cited By :26 Export Date: 30 January 2023 Correspondence Address: Amarir, F.; Laboratory of Parasitology, Department of Parasitology, National Institute of Hygiene, Agdal, Rabat, Morocco; email: Fatima_inh@yahoo.fr Funding details: World Health Organization, WHO, 2 008/237-0 Funding text 1: This study was supported by the World Health Organization (Reference no. 2 008/237-0). 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Abassi, I., Charles, K., Robert, F.S., Curtis, K., Eric, M., Joseph, H., Differentiation of Schistosoma haematobium from related schistosomes by PCR amplifying an inter repeat sequence (2007) Am J Trop Med Hyg, 76, pp. 950-955. , 17488921; Melo, F.L., Gomes, A.L.D.V., Barbosa, C.S., Werkhauser, R.P., Abath, F.G.C., Development of molecular approaches for the identification of transmission sites of schistosomiasis (2006) Transactions of the Royal Society of Tropical Medicine and Hygiene, 100 (11), pp. 1049-1055. , DOI 10.1016/j.trstmh.2005.12.008, PII S0035920306000381; Lambrechts, L., Fellous, S., Koella, J.C., Coevolutionary interactions between host and parasite genotypes (2006) Trends in Parasitology, 22 (1), pp. 12-16. , DOI 10.1016/j.pt.2005.11.008, PII S1471492205003144; Rapport d'activité annuel des programmes de lutte contre les maladies parasitaires (2003) Bull Épidémiologique, 1, p. 25. , Ministry of Health Direction of Epidemiology control of Diseases Morocco; Rapport d'activité annuel des programmes de lutte contre les maladies parasitaires (2004) Bull Épidémiologique, 1, p. 31. , Ministry of Health Direction of Epidemiology control of Diseases Morocco; Rapport d'activité annuel des programmes de lutte contre les maladies parasitaires (2001) Bull Épidémiologique, 1, p. 31. , Ministry of Health Direction of Epidemiology control of Diseases Morocco; Rapport d'activité annuel des programmes de lutte contre les maladies parasitaires (2000) Bull Épidémiologique, 1, pp. 41-42. , Ministry of Health Direction of Epidemiology control of Diseases Morocco; Moussa, A.H., Abou El-Hassan, A.A., The effect of water temperature on the snail intermediate hosts of schistosomiasis in Egypt (1972) Egyptian Med Soc, 55, pp. 148-165; Hamburger, J., He-Na, Abbasi, I., Ramzy, R.M., Jourdane, J., Ruppel, A., Polymerase chain reaction assay based on a highly repeated sequence of Schistosoma haematobium: A potential tool for monitoring schistosome-infested water (2001) American Journal of Tropical Medicine and Hygiene, 65 (6), pp. 907-911; Hamburger, J., He-Na, Xu, Y.X., Ramzy, R.M., Jourdane, J., Ruppel, A., A polymerase chain reaction assay for detecting snails infected with Bilharzia parasites (Schistosoma mansoni) from very early prepatency (1998) American Journal of Tropical Medicine and Hygiene, 59 (6), pp. 872-876; Christensen, N.O., Mutani, A., Frandsen, F., A review of the biology and transmission ecology of African bovine species of the genus Schistosoma (1983) Zeitschrift fur Parasitenkunde, 69 (5), pp. 551-570. , DOI 10.1007/BF00926667; Mone, H., Mowahid, G., Monard, S., The distribution of Schistosoma bovis Sonsino, 1876 in relation to intermediate host mollusc-parasite relationship (2000) Adv Parasitol, 44, pp. 100-138; Vercruysse, J., Southgate, V.R., Rollinson, D., De Clercq, D., Sacko, M., De Bont, J., Mungomba, L.M., Studies on transmission and schistosome interactions in Senegal, Mali and Zambia (1994) Tropical and Geographical Medicine, 46 (4), pp. 220-226; Jourdane, J., Southgate, V.R., Pages, J.R., Durand, P., Tchuem Tchuente, L.A., Recent Studies on Schistosoma intercalatum: Taxonomic Status, Puzzling Distribution and Transmission Foci Revisited (2001) Memorias do Instituto Oswaldo Cruz, 96 (SUPPL.), pp. 45-48; Webster, B.L., Rollinson, D., Stothard, J.R., Huyse, T., Rapid diagnostic multiplex PCR (RD-PCR) to discriminate Schistosoma haematobium and S. Bovis (2010) J Helminthol, 84, pp. 107-114. , 10.1017/S0022149X09990447 19646307; Abbasi, I., Hamburger, J., Kariuki, C., Mungai, P.L., Muchiri, E.M., King, C.H., Differentiating Schistosoma haematobium from Related Animal Schistosomes by PCR. Amplifying Inter-Repeat Sequences Flanking Newly Selected Repeated Sequences (2012) Am J Trop Med Hyg, 87 (6), pp. 1059-1064. , 10.4269/ajtmh.2012.12-0243 23109375; Huyse, T., Webster, B.L., Geldof, S., Stothard, R.J., Diaw, O.T., Polman, K., Rollinson, D., Bidirectional introgressive hybridization between a cattle and human schistosome species (2009) PLoS Pathog, 5 (9), p. 51000571. , 10.1371/journal.ppat.1000571 19730700; Elimination of schistosomiasis from low-transmission areas (2008) Report of Who Informal Consultation, pp. 1-21. , WHO Salvador, Brazil; Sturrock, R.F., Snail collection to detect schistosome transmission sites (1986) Parasitol Today, 2, pp. 59-63. , 10.1016/0169-4758(86)90155-9 15462771; Yacoubi, B., Zekhnini, A., Moukrim, A., Rondelaud, D., Bulins, planorbes et endémie bilharzienne dans le sud-ouest marocain (2007) Bull Soc Pathol Exot, 100, pp. 174-175. , 17824308; Zhou, Y.B., Liang, S., Jiang, Q.W., Factors impacting on progress towards elimination of transmission of schistosomiasis japonica in China (2012) Parasites Vectors, 5, p. 275. , 10.1186/1756-3305-5-275 23206326; Hua, H.Y., Wang, W., Cao, G.Q., Tang, F., Liang, Y.S., Improving the management of imported schistosomiasis haematobia in China: Lessons from a case with multiple misdiagnoses (2013) Parasites Vectors, 6, p. 260. , 10.1186/1756-3305-6-260 24020375; Glenn, C.T., Lance, S.L., McKee, A.M., Webster, B.L., Emery, A.M., Zerlotini, A., Oliveira, G., Faircloth, B.C., Significant variance in genetic diversity among populations of Schistosoma haematobium detected using microsatellite DNA loci from a genome-wide database (2013) Parasites Vectors, 6, p. 300. , 10.1186/1756-3305-6-300 24499537; Utzinger, J., N'Goran, E.K., Caffreye, C.R., Rkeiser, J., From innovation to application: Social-ecological context, diagnostics, drugs and integrated control of schistosomiasis (2011) Acta Trop, 120, pp. 121-137

PY - 2014

Y1 - 2014

N2 - Background: This is the first study in Morocco to estimate snail infection rates at the last historic transmission sites of schistosomiasis, known to be free from new infection among humans since 2004. Screening of large numbers of snails for infection is one way to confirm that Schistosoma haematobium transmission has stopped and does not resurge. Methods. A total of 2703 Bulinus truncatus snails were collected from 24 snail habitats in five provinces of Morocco: Errachidia, El Kelaa des Sraghna, Tata, Beni Mellal, and Chtouka Ait Baha. All visible snails were collected with a scoop net or by hand. We used waders and gloves as simple precautions. Snails were morphologically identified according to Moroccan Health Ministry guide of schistosomiasis (1982).All snails were analyzed in pools by molecular tool, using primers from the newly identified repeated DNA sequence, termed DraI, in the S. haematobium group. To distinguish S. bovis and S. haematobium, the snails were analyzed by Sh110/Sm-Sl PCR that was specific of S. haematobium. Results: The results showed that snails from Errachidia, Chtouka Ait Baha, sector of Agoujgal in Tata and sector of Mbarkiya in El kelaa des Sraghna were negative for DraI PCR; but, snails from remaining snail habitats of El Kelaa des Sraghna, Tata and Beni Mellal were positive. This led to suggest the presence of circulating schistosome species (S. haematobium, S. bovis or others) within these positive snail habitats. Subsequently, confirmation with S. haematobium species specific molecular assay, Sh110/Sm-Sl PCR, showed that none of the collected snails were infected by S. haematobium in all historic endemic areas. Conclusion: The absence of S. haematobium infection in snails supports the argument of S. haematobium transmission interruption in Morocco. © 2014 Amarir et al.; licensee BioMed Central Ltd.

AB - Background: This is the first study in Morocco to estimate snail infection rates at the last historic transmission sites of schistosomiasis, known to be free from new infection among humans since 2004. Screening of large numbers of snails for infection is one way to confirm that Schistosoma haematobium transmission has stopped and does not resurge. Methods. A total of 2703 Bulinus truncatus snails were collected from 24 snail habitats in five provinces of Morocco: Errachidia, El Kelaa des Sraghna, Tata, Beni Mellal, and Chtouka Ait Baha. All visible snails were collected with a scoop net or by hand. We used waders and gloves as simple precautions. Snails were morphologically identified according to Moroccan Health Ministry guide of schistosomiasis (1982).All snails were analyzed in pools by molecular tool, using primers from the newly identified repeated DNA sequence, termed DraI, in the S. haematobium group. To distinguish S. bovis and S. haematobium, the snails were analyzed by Sh110/Sm-Sl PCR that was specific of S. haematobium. Results: The results showed that snails from Errachidia, Chtouka Ait Baha, sector of Agoujgal in Tata and sector of Mbarkiya in El kelaa des Sraghna were negative for DraI PCR; but, snails from remaining snail habitats of El Kelaa des Sraghna, Tata and Beni Mellal were positive. This led to suggest the presence of circulating schistosome species (S. haematobium, S. bovis or others) within these positive snail habitats. Subsequently, confirmation with S. haematobium species specific molecular assay, Sh110/Sm-Sl PCR, showed that none of the collected snails were infected by S. haematobium in all historic endemic areas. Conclusion: The absence of S. haematobium infection in snails supports the argument of S. haematobium transmission interruption in Morocco. © 2014 Amarir et al.; licensee BioMed Central Ltd.

KW - Bulinus truncatus

KW - DraI PCR

KW - Elimination

KW - Morocco

KW - Schistosoma haematobium

KW - Schistosomiasis

KW - Sh110/Sm-Sl PCR

KW - repetitive DNA

KW - article

KW - controlled study

KW - DNA extraction

KW - habitat

KW - microorganism detection

KW - nonhuman

KW - parasite transmission

KW - polymerase chain reaction

KW - Schistosoma hematobium

KW - schistosomiasis

KW - sensitivity and specificity

KW - snail

KW - animal

KW - isolation and purification

KW - parasitology

KW - procedures

KW - schistosomiasis haematobia

KW - transmission

KW - Animals

KW - Polymerase Chain Reaction

KW - Schistosomiasis haematobia

KW - Sensitivity and Specificity

KW - Snails

UR - http://www.scopus.com/inward/record.url?scp=84904693257&partnerID=8YFLogxK

U2 - 10.1186/1756-3305-7-288

DO - 10.1186/1756-3305-7-288

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

SN - 1756-3305

VL - 7

JO - Parasites and Vectors

JF - Parasites and Vectors

IS - 1

M1 - 288

ER -

Schistosoma haematobium detection in snails by DraI PCR and Sh110/Sm-Sl PCR: Further evidence of the interruption of schistosomiasis transmission in Morocco: Further evidence of the interruption of schistosomiasis transmission in Morocco

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