Scrapie-like prion protein accumulates in aggresomes of cyclosporin A-treated cells

Ehud Cohen, Albert Taraboulos*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

91 Scopus citations


Prion diseases are infectious, sporadic and inherited fatal neurodegenerations that are propagated by an abnormal refolding of the cellular prion protein PrPC. Which chaperones assist the normal folding of PrPC is unknown. The linkage of familial Gerstmann-Sträussler-Scheinker (GSS) syndrome with proline substitutions in PrP raised the prospect that peptidylprolyl cis-trans isomerases (PPIases) may play a role in normal PrP metabolism. Here we used cyclosporin A (CsA), an immunosuppressant, to inhibit the cyclophilin family of PPIases in cultured cells. CsA-treated cells accumulated proteasome-resistant, 'prion-like' PrP species, which deposited in long-lived aggresomes. PrP aggresomes also formed with disease-linked proline mutants when proteasomes were inhibited. These results suggest mechanisms whereby abnormally folded cytosolic PrP may in some cases participate in the development of spontaneous and inherited prion diseases.

Original languageAmerican English
Pages (from-to)404-417
Number of pages14
JournalEMBO Journal
Issue number3
StatePublished - 3 Feb 2003


  • Aggresome
  • Cyclophilin
  • Cyclosporin A
  • Encephalopathy
  • Prion protein


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