TY - JOUR
T1 - Screening of the antioxidant and nutritional properties, phenolic contents and proteins of five durian cultivars
AU - Toledo, Fernando
AU - Arancibia-Avila, Patricia
AU - Park, Yong Seo
AU - Jung, Soon Teck
AU - Kang, Seong Gook
AU - Gu Heo, Buk
AU - Drzewiecki, Jerzy
AU - Zachwieja, Zofia
AU - Zagrodzki, Pawel
AU - Pasko, Pawel
AU - Gorinstein, Shela
PY - 2008/8
Y1 - 2008/8
N2 - The antioxidant activities of different durian cultivars at the same stage of ripening (Mon Thong, Chani, Kan Yao, Pung Manee and Kradum) were compared in order to choose the best as a supplement in the human diet. Total polyphenols (mg gallic acid equivalent/100 g fresh weight (FW)) and flavonoids (mg catechin equivalent (CE)/100 g FW) in Mon Thong (361.4 ± 23.2 and 93.9 ± 7.4) were significantly higher (P > 0.05) than in Kradum (271.5 ± 11.2 and 69.2 ± 5.3) and Kan Yao (283.2 ± 16.5 and 72.1 ± 6.8). The free polyphenols and flavonoids showed lower results than the hydrolyzed ones. Anthocyanins (μg cyanidin-3-glucoside equivalent/100 g FW) and flavanols (μg CE/100 g FW) were significantly higher in Mon Thong (427.3 ± 23.8 and 171.4 ± 16.3) than in Kradum (320.2 ± 12.1 and 128.6 ± 9.7) and Kan Yao (335.3 ± 14.1 and 134.4 ± 11.7). Ultraviolet spectroscopy and high-performance liquid chromatography/diode array detection analyses showed that caffeic acid and quercetin were the dominant bioactive substances in Mon Thong cultivar. The antioxidant activity (μM trolox equivalent/100 g FW) of Mon Thong cultivar (260.8 ± 20.2, 1,075.6 ± 81.4 and 2,352.7 ± 124.2) determined by ferric-reducing/antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) with Trolox equivalent antioxidant capacity (TEAC) assays was significantly higher (P > 0.05) than in Kradum (197.4 ± 8.9, 806.5 ± 31.2 and 1,773.2 ± 102.5) and in Kan Yao (204.7 ± 9.7, 845.5 ± 48.6 and 1,843.6 ± 107.5). The correlation coefficients between polyphenols, flavonoids, flavanols and FRAP, CUPRAC and TEAC capacities were between 0.89 and 0.98. In extracted and separated by electrophoresis durian proteins, some differences were found in the sodium dodecyl sulfate-protein bands in the region of 16 and 68 kDa for Kradum, 45 kDa for Mon Thong and three bands for Kan Yao. Antioxidants and proteins can be used for characterization of the quality of durian cultivars. In conclusion, the bioactivity of durian cultivars Mon Thong, Chani and Pung Manee was high and the total polyphenols were the main contributors to the overall antioxidant capacity. The results of our investigation in vitro are comparable with other fruits that widely used in human diets. Therefore, Durian can be used as a supplement for nutritional and healthy purposes, especially Durian Mon Thong, Chani and Pung Manee.
AB - The antioxidant activities of different durian cultivars at the same stage of ripening (Mon Thong, Chani, Kan Yao, Pung Manee and Kradum) were compared in order to choose the best as a supplement in the human diet. Total polyphenols (mg gallic acid equivalent/100 g fresh weight (FW)) and flavonoids (mg catechin equivalent (CE)/100 g FW) in Mon Thong (361.4 ± 23.2 and 93.9 ± 7.4) were significantly higher (P > 0.05) than in Kradum (271.5 ± 11.2 and 69.2 ± 5.3) and Kan Yao (283.2 ± 16.5 and 72.1 ± 6.8). The free polyphenols and flavonoids showed lower results than the hydrolyzed ones. Anthocyanins (μg cyanidin-3-glucoside equivalent/100 g FW) and flavanols (μg CE/100 g FW) were significantly higher in Mon Thong (427.3 ± 23.8 and 171.4 ± 16.3) than in Kradum (320.2 ± 12.1 and 128.6 ± 9.7) and Kan Yao (335.3 ± 14.1 and 134.4 ± 11.7). Ultraviolet spectroscopy and high-performance liquid chromatography/diode array detection analyses showed that caffeic acid and quercetin were the dominant bioactive substances in Mon Thong cultivar. The antioxidant activity (μM trolox equivalent/100 g FW) of Mon Thong cultivar (260.8 ± 20.2, 1,075.6 ± 81.4 and 2,352.7 ± 124.2) determined by ferric-reducing/antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) with Trolox equivalent antioxidant capacity (TEAC) assays was significantly higher (P > 0.05) than in Kradum (197.4 ± 8.9, 806.5 ± 31.2 and 1,773.2 ± 102.5) and in Kan Yao (204.7 ± 9.7, 845.5 ± 48.6 and 1,843.6 ± 107.5). The correlation coefficients between polyphenols, flavonoids, flavanols and FRAP, CUPRAC and TEAC capacities were between 0.89 and 0.98. In extracted and separated by electrophoresis durian proteins, some differences were found in the sodium dodecyl sulfate-protein bands in the region of 16 and 68 kDa for Kradum, 45 kDa for Mon Thong and three bands for Kan Yao. Antioxidants and proteins can be used for characterization of the quality of durian cultivars. In conclusion, the bioactivity of durian cultivars Mon Thong, Chani and Pung Manee was high and the total polyphenols were the main contributors to the overall antioxidant capacity. The results of our investigation in vitro are comparable with other fruits that widely used in human diets. Therefore, Durian can be used as a supplement for nutritional and healthy purposes, especially Durian Mon Thong, Chani and Pung Manee.
KW - Antioxidant activity
KW - Bioactive compounds
KW - Cultivars
KW - Durian
KW - Proteins
UR - http://www.scopus.com/inward/record.url?scp=48249134094&partnerID=8YFLogxK
U2 - 10.1080/09637480701603082
DO - 10.1080/09637480701603082
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C2 - 18979619
AN - SCOPUS:48249134094
SN - 0963-7486
VL - 59
SP - 415
EP - 427
JO - International Journal of Food Sciences and Nutrition
JF - International Journal of Food Sciences and Nutrition
IS - 5
ER -