Scribble, Lgl1, and myosin IIA interact with α-/β-catenin to maintain epithelial junction integrity

Maha Abedrabbo, Shirel Sloomy, Reham Abu-Leil, Einav Kfir-Cohen, Shoshana Ravid*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

E-cadherin-catenin complex together with the cytoskeleton, builds the core of Adherens junctions (AJs). It has been reported that Scribble stabilizes the coupling of E-cadherin with catenins promoting epithelial cell adhesion, but the mechanism remains unknown. We show that Scribble, Lgl1, and NMII-A reside in a complex with E-cadherin-catenin complex. Depletion of either Scribble or Lgl1 disrupts the localization of E-cadherin-catenin complex to AJs. aPKCζ phosphorylation of Lgl1 regulates AJ localization of Lgl1 and E-cadherin-catenin complexes. Both Scribble and Lgl1 regulate the activation and recruitment of NMII-A at AJs. Finally, Scribble and Lgl1 are downregulated by TGFβ-induced EMT, and their re-expression during EMT impedes its progression. Our results provide insight into the mechanism regulating AJ integrity by Scribble, Lgl1, and NMII-A.

Original languageAmerican English
Pages (from-to)1-23
Number of pages23
JournalCell Adhesion and Migration
Volume17
Issue number1
DOIs
StatePublished - Dec 2023

Bibliographical note

Publisher Copyright:
© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

Keywords

  • Adherens junction
  • EMT
  • cell adhesion
  • cell polarity
  • myosin
  • tumor suppressor

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