Selective Extraction of Biomolecules Using a Bidirectional Flow Filter

Vesna Bacheva, Federico Paratore*, Maya Bar-Dolev, Baruch Rofman, Govind V. Kaigala*, Moran Bercovici*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

We present a microfluidic device for selective separation and extraction of molecules based on their diffusivity. The separation relies on electroosmotically driven bidirectional flows in which high-diffusivity species experience a net-zero velocity and lower diffusivity species are advected to a collection reservoir. The device can operate continuously and is suitable for processing low sample volumes. Using several model systems, we show that the extraction efficiency of the system is maintained at more than 90% over tens of minutes with a purity of more than 99%. We demonstrate the applicability of the device to the extraction of genomic DNA from short DNA fragments.

Original languageAmerican English
Pages (from-to)10584-10588
Number of pages5
JournalAnalytical Chemistry
Volume94
Issue number30
DOIs
StatePublished - 2 Aug 2022
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2022 American Chemical Society.

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