Selective Extraction of Biomolecules Using a Bidirectional Flow Filter

Vesna Bacheva; Federico Paratore; Maya Bar-Dolev; Baruch Rofman; Govind V. Kaigala; Moran Bercovici

doi:10.1021/acs.analchem.2c01074

Selective Extraction of Biomolecules Using a Bidirectional Flow Filter

Vesna Bacheva, Federico Paratore^*, Maya Bar-Dolev, Baruch Rofman, Govind V. Kaigala^*, Moran Bercovici^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

We present a microfluidic device for selective separation and extraction of molecules based on their diffusivity. The separation relies on electroosmotically driven bidirectional flows in which high-diffusivity species experience a net-zero velocity and lower diffusivity species are advected to a collection reservoir. The device can operate continuously and is suitable for processing low sample volumes. Using several model systems, we show that the extraction efficiency of the system is maintained at more than 90% over tens of minutes with a purity of more than 99%. We demonstrate the applicability of the device to the extraction of genomic DNA from short DNA fragments.

Original language	English
Pages (from-to)	10584-10588
Number of pages	5
Journal	Analytical Chemistry
Volume	94
Issue number	30
DOIs	https://doi.org/10.1021/acs.analchem.2c01074
State	Published - 2 Aug 2022
Externally published	Yes

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Publisher Copyright:
© 2022 American Chemical Society.

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abstract = "We present a microfluidic device for selective separation and extraction of molecules based on their diffusivity. The separation relies on electroosmotically driven bidirectional flows in which high-diffusivity species experience a net-zero velocity and lower diffusivity species are advected to a collection reservoir. The device can operate continuously and is suitable for processing low sample volumes. Using several model systems, we show that the extraction efficiency of the system is maintained at more than 90% over tens of minutes with a purity of more than 99%. We demonstrate the applicability of the device to the extraction of genomic DNA from short DNA fragments.",

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AU - Rofman, Baruch

AU - Kaigala, Govind V.

AU - Bercovici, Moran

PY - 2022/8/2

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AB - We present a microfluidic device for selective separation and extraction of molecules based on their diffusivity. The separation relies on electroosmotically driven bidirectional flows in which high-diffusivity species experience a net-zero velocity and lower diffusivity species are advected to a collection reservoir. The device can operate continuously and is suitable for processing low sample volumes. Using several model systems, we show that the extraction efficiency of the system is maintained at more than 90% over tens of minutes with a purity of more than 99%. We demonstrate the applicability of the device to the extraction of genomic DNA from short DNA fragments.

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Selective Extraction of Biomolecules Using a Bidirectional Flow Filter

Abstract

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