TY - JOUR
T1 - Selective flexible packaging pathways of the segmented genome of influenza A virus
AU - Haralampiev, Ivan
AU - Prisner, Simon
AU - Nitzan, Mor
AU - Schade, Matthias
AU - Jolmes, Fabian
AU - Schreiber, Max
AU - Loidolt-Krüger, Maria
AU - Jongen, Kalle
AU - Chamiolo, Jasmine
AU - Nilson, Niklaas
AU - Winter, Franziska
AU - Friedman, Nir
AU - Seitz, Oliver
AU - Wolff, Thorsten
AU - Herrmann, Andreas
N1 - Publisher Copyright:
© 2020, The Author(s).
PY - 2020/12/1
Y1 - 2020/12/1
N2 - The genome of influenza A viruses (IAV) is encoded in eight distinct viral ribonucleoproteins (vRNPs) that consist of negative sense viral RNA (vRNA) covered by the IAV nucleoprotein. Previous studies strongly support a selective packaging model by which vRNP segments are bundling to an octameric complex, which is integrated into budding virions. However, the pathway(s) generating a complete genome bundle is not known. We here use a multiplexed FISH assay to monitor all eight vRNAs in parallel in human lung epithelial cells. Analysis of 3.9 × 105 spots of colocalizing vRNAs provides quantitative insights into segment composition of vRNP complexes and, thus, implications for bundling routes. The complexes rarely contain multiple copies of a specific segment. The data suggest a selective packaging mechanism with limited flexibility by which vRNPs assemble into a complete IAV genome. We surmise that this flexibility forms an essential basis for the development of reassortant viruses with pandemic potential.
AB - The genome of influenza A viruses (IAV) is encoded in eight distinct viral ribonucleoproteins (vRNPs) that consist of negative sense viral RNA (vRNA) covered by the IAV nucleoprotein. Previous studies strongly support a selective packaging model by which vRNP segments are bundling to an octameric complex, which is integrated into budding virions. However, the pathway(s) generating a complete genome bundle is not known. We here use a multiplexed FISH assay to monitor all eight vRNAs in parallel in human lung epithelial cells. Analysis of 3.9 × 105 spots of colocalizing vRNAs provides quantitative insights into segment composition of vRNP complexes and, thus, implications for bundling routes. The complexes rarely contain multiple copies of a specific segment. The data suggest a selective packaging mechanism with limited flexibility by which vRNPs assemble into a complete IAV genome. We surmise that this flexibility forms an essential basis for the development of reassortant viruses with pandemic potential.
UR - http://www.scopus.com/inward/record.url?scp=85089975241&partnerID=8YFLogxK
U2 - 10.1038/s41467-020-18108-1
DO - 10.1038/s41467-020-18108-1
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C2 - 32859915
AN - SCOPUS:85089975241
SN - 2041-1723
VL - 11
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 4355
ER -
