TY - JOUR
T1 - Selective translation of leaderless mRNAs by specialized ribosomes generated by MazF in Escherichia coli
AU - Vesper, Oliver
AU - Amitai, Shahar
AU - Belitsky, Maria
AU - Byrgazov, Konstantin
AU - Kaberdina, Anna Chao
AU - Engelberg-Kulka, Hanna
AU - Moll, Isabella
PY - 2011/9/30
Y1 - 2011/9/30
N2 - Escherichia coli (E. coli) mazEF is a stress-induced toxin-antitoxin (TA) module. The toxin MazF is an endoribonuclease that cleaves single-stranded mRNAs at ACA sequences. Here, we show that MazF cleaves at ACA sites at or closely upstream of the AUG start codon of some specific mRNAs and thereby generates leaderless mRNAs. Moreover, we provide evidence that MazF also targets 16S rRNA within 30S ribosomal subunits at the decoding center, thereby removing 43 nucleotides from the 3′ terminus. As this region comprises the anti-Shine-Dalgarno (aSD) sequence that is required for translation initiation on canonical mRNAs, a subpopulation of ribosomes is formed that selectively translates the described leaderless mRNAs both in vivo and in vitro. Thus, we have discovered a modified translation machinery that is generated in response to MazF induction and that probably serves for stress adaptation in Escherichia coli.
AB - Escherichia coli (E. coli) mazEF is a stress-induced toxin-antitoxin (TA) module. The toxin MazF is an endoribonuclease that cleaves single-stranded mRNAs at ACA sequences. Here, we show that MazF cleaves at ACA sites at or closely upstream of the AUG start codon of some specific mRNAs and thereby generates leaderless mRNAs. Moreover, we provide evidence that MazF also targets 16S rRNA within 30S ribosomal subunits at the decoding center, thereby removing 43 nucleotides from the 3′ terminus. As this region comprises the anti-Shine-Dalgarno (aSD) sequence that is required for translation initiation on canonical mRNAs, a subpopulation of ribosomes is formed that selectively translates the described leaderless mRNAs both in vivo and in vitro. Thus, we have discovered a modified translation machinery that is generated in response to MazF induction and that probably serves for stress adaptation in Escherichia coli.
UR - https://www.scopus.com/pages/publications/80053563668
U2 - 10.1016/j.cell.2011.07.047
DO - 10.1016/j.cell.2011.07.047
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C2 - 21944167
AN - SCOPUS:80053563668
SN - 0092-8674
VL - 147
SP - 147
EP - 157
JO - Cell
JF - Cell
IS - 1
ER -