Self-assembling elastin-like peptides growth factor chimeric nanoparticles for the treatment of chronic wounds

Piyush Koria, Hiroshi Yagi, Yuko Kitagawa, Zaki Megeed, Yaakov Nahmias, Robert Sheridan, Martin L. Yarmush

Research output: Contribution to journalArticlepeer-review

156 Scopus citations

Abstract

Chronic wounds are associated with poor epidermal and dermal remodeling. Previous work has shown the efficacy of keratinocyte growth factor (KGF) in reepithelialization and elastin in dermal wound healing. Here we demonstrate the fabrication of a fusion protein comprising of elastin-like peptides and KGF. This fusion protein retains the performance characteristics of KGF and elastin as evidenced by its enhancement of keratinocyte and fibroblast proliferation. It also preserved the characteristic elastin-like peptides inverse phase transitioning allowing the recombinant protein to be expressed in bacterial hosts (such as Escherichia coli) and purified rapidly and easily using inverse temperature cycling. The fusion protein self-assembled into nanoparticles at physiological temperatures. Whenapplied to full thickness,woundsin Lepr db diabetic mice these particles enhanced reepithelialization and granulation, by 2- and 3-fold respectively, when compared to the controls. The data strongly suggests that these self-assembled nanoparticles may be beneficial in the treatment of chronic wounds resulting from diabetes or other underlying circulatory conditions.

Original languageAmerican English
Pages (from-to)1034-1039
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number3
DOIs
StatePublished - 18 Jan 2011

Keywords

  • Fusion peptides
  • Regeneration
  • Skin

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