Self-assembly of enzymes on dna scaffolds: en route to biocatalytic cascades and the synthesis of metallic nanowires

Ofer I. Wilner, Simcha Shimron, Yossi Weizmann, Zhen Gang Wang, Itamar Willner

Research output: Contribution to journalArticlepeer-review

125 Scopus citations

Abstract

DNA strands consisting of programmed sequence-specific domains were synthesized by the rolling circle amplification (RCA) process. The spatial positioning of glucose oxidase (GOx) and of horseradish peroxidase (HRP) on the RCA-synthesized DNA template via hybridization enabled the activation of the bienzyme cascade. The GOx-catalyzed oxidation of glucose yielded gluconic acid and H2O2, and the resulting H2O2 oxidized 2,2'-azino-bis[3-ethylbenzthiazoline-6-sulfonic-acid] (ABTS 2-) in the presence of HRP. The enzyme cascade could not be activated in the absence of the organizing DNA template or in the presence of a foreign DNA. Also, Au NPs-functionalized GOx was hybridized with the RCA-synthesized single-stranded DNA. The biocatalytic growth of the NPs through the oxidation of glucose, in the presence of AuCl4-, enabled the synthesis of 1-5 μm long Au wires, exhibiting a width of ca. 150 nm.

Original languageEnglish
Pages (from-to)2040-2043
Number of pages4
JournalNano Letters
Volume9
Issue number5
DOIs
StatePublished - 13 May 2009

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