Sensitive detection and identification of DNA and RNA using a patterned capillary tube

Amos Bardea, Noa Burshtein, Yinon Rudich, Tomer Salame, Carmit Ziv, Oded Yarden, Ron Naaman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

We report on a new ultrasensitive and fast technique for the detection and identification of both DNA and RNA with sensitivity of a few molecules. The new method is based on a patterned capillary tube (PCT) in which the internal surface of a glass tube is patterned with rings of different single-stranded DNA probes. A solution containing single-stranded analyte flows through the tube. Upon hybridization of appropriate DNA and RNA from the solution, DNA polymerase and reverse transcriptase (RT) are employed to synthesize the complementary nucleic acids with deoxynucleoside triphosphate (dNTP) labeled with fluorophores. The sample-analyte hybrids are detected by their fluorescence signal. We show that the new method is sensitive, is specific, can detect simultaneously both DNA and RNA from the same sample, and allows detection of analytes in serum.

Original languageAmerican English
Pages (from-to)9418-9423
Number of pages6
JournalAnalytical Chemistry
Volume83
Issue number24
DOIs
StatePublished - 15 Dec 2011

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