Sensitivity of Haloquadratum and Salinibacter to antibiotics and other inhibitors: Implications for the assessment of the contribution of Archaea and Bacteria to heterotrophic activities in hypersaline environments

Rahel Elevi Bardavid, Aharon Oren*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Antibiotics and bile salts have been used to differentiate between heterotrophic activity of halophilic Archaea and Bacteria in saltern ponds. In NaCl-saturated brines of crystallizer ponds, most activity was attributed to Archaea. Following the recent isolation of Haloquadratum, the dominant archaeon in the salterns (reported to be sensitive to chloramphenicol and erythromycin), and the discovery of Salinibacter, a representative of the Bacteria, in the same ecosystem, reevaluation of the earlier data is required. The authors measured amino acid incorporation by Haloquadratum and Salinibacter suspended in crystallizer brine to investigate the suitability of antibiotics and bile salts to distinguish between archaeal and bacterial activities. The amino acid uptake rate per cell in Salinibacter was two orders of magnitude lower than that of Haloquadratum under the same conditions. Salinibacter was inhibited by chloramphenicol, erythromycin, and deoxycholate, but not by taurocholate. Erythromycin did not inhibit incorporation by Haloquadratum, but moderate inhibition was found by chloramphenicol at 10-50 μg mL-1. Deoxycholate was highly inhibitory, but only partial inhibition was obtained in the presence of 25 μg mL-1 taurocholate. Inhibition by chloramphenicol and taurocholate increased with increasing salt concentration. Erythromycin and taurocholate proved most valuable to differentiate between archaeal and bacterial activities in saltern brines.

Original languageAmerican English
Pages (from-to)309-315
Number of pages7
JournalFEMS Microbiology Ecology
Volume63
Issue number3
DOIs
StatePublished - Mar 2008

Keywords

  • Bile salts
  • Chloramphenicol
  • Erythromycin
  • Haloquadratum walsbyi
  • Salinibacter ruber
  • Salterns

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